LOCUS       sequence-138283-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK294 (mAID-mCherry2-Bsr)
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgagcagc
       61 tgaagcttac cgctagcatg aaaacattta acatttctca acaagatcta gaattagtag
      121 aagtagcgac agagaagatt acaatgcttt atgaggataa taaacatcat gtgggagcgg
      181 caattcgtac gaaaacagga gaaatcattt cggcagtaca tattgaagcg tatataggac
      241 gagtaactgt ttgtgcagaa gccattgcga ttggtagtgc agtttcgaat ggacaaaagg
      301 attttgacac gattgtagct gttagacacc cttattctga cgaagtagat agaagtattc
      361 gagtggtaag tccttgtggt atgtgtaggg agttgatttc agactatgca ccagattgtt
      421 ttgtgttaat agaaatgaat ggcaagttag tcaaaactac gattgaagaa ctcattccac
      481 tcaaatatac ccgaaattaa gctagccgcc cgccccacga cccgcagcgc ccgaccgaaa
      541 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
      601 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
      661 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
      721 gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata
      781 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
      841 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
      901 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
      961 gaacgagatc agcagcctct gttccaca
//
