LOCUS       sequence-138285-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mAID-mClover using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK290 (mAID-mClover-Hygro)
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta gatctcgagc
       61 agctgaagct taccgctagc atggatagat ccggaaagcc tgaactcacc gcgacgtctg
      121 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
      181 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
      241 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
      301 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
      361 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
      421 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
      481 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
      541 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
      601 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
      661 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
      721 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
      781 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
      841 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
      901 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
      961 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgcc
//
