LOCUS       sequence-138288-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GST tagged protein for antibody production screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, 
            Matthews G
  TITLE     A novel cyclic nucleotide-gated ion channel enriched in synaptic 
            terminals of isotocin neurons in zebrafish brain and pituitary.
  JOURNAL   Neuroscience. 2010 Jan 13;165(1):79-89. doi: 
            10.1016/j.neuroscience.2009.09.040. Epub 2009 Sep 22.
  PUBMED    19778592
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuroscience.2009.09.040"; journalName: "Neuroscience"; 
            date: "2010-01-13- 13"; volume: "165"; issue: "1"; pages: "79-89"
COMMENT     Sequence Label: pGEX1ZFCNGA5
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(185..190,191..208,209..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    223..239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     247..263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             259..786
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(279..296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(279..295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(288..310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(888..893,894..909,910..916)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    924..940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
       61 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      121 tggaaagcgg gcagtgagcg caaccaatta atgtaagtta gctcactcat taggcacccc
      181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      241 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
      301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      421 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      481 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
      541 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
      601 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
      661 ctacaggaag gccagacgcg aattattttt gatggcgttg gaattagctt atcgactgca
      721 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
      781 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
      841 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
      901 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagtat
      961 tcatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
     1021 ttttggaata tcttgaagaa aa
//
