LOCUS       sequence-138291-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His tagged protein for antibody production screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, 
            Matthews G
  TITLE     A novel cyclic nucleotide-gated ion channel enriched in synaptic 
            terminals of isotocin neurons in zebrafish brain and pituitary.
  JOURNAL   Neuroscience. 2010 Jan 13;165(1):79-89. doi: 
            10.1016/j.neuroscience.2009.09.040. Epub 2009 Sep 22.
  PUBMED    19778592
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuroscience.2009.09.040"; journalName: "Neuroscience"; 
            date: "2010-01-13- 13"; volume: "165"; issue: "1"; pages: "79-89"
COMMENT     Sequence Label: pet28bZFCNGA5
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(47..502)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(184..205)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     396..415
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(539..586)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     582..600
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(653..670)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
       61 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
      241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
      301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
      361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
      421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
      481 aatgcgccgc tacagggcgc gtcccattcg ccaatccgga tatagttcct cctttcagca
      541 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
      601 tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt
      661 ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga gctcgaattc catgcttagc
      721 acttaaaggt gaatgttgac ctcactgttt gtaccatccc catcaatagt gctttcatta
      781 ccatcagaaa ggaatccact gccagaatga ttggttctgc attccagagt ggtaagtctc
      841 tgcttcaggt gctgctgagt tgctgtatat tgagccaata gtcgagcaaa tcgagtttgt
      901 aatatttcaa tagtatcttc catcctctct actcttttct caatatcttc cacccctatt
      961 ccgtctgcct ccacctcctc gagcaaaccc tcttttataa gaatttgtct tcccttctct
     1021 tccagaactt tcttagcatc tggatcccga cc
//
