LOCUS       sequence-138292-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His tagged protein for antibody production screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, 
            Matthews G
  TITLE     A novel cyclic nucleotide-gated ion channel enriched in synaptic 
            terminals of isotocin neurons in zebrafish brain and pituitary.
  JOURNAL   Neuroscience. 2010 Jan 13;165(1):79-89. doi: 
            10.1016/j.neuroscience.2009.09.040. Epub 2009 Sep 22.
  PUBMED    19778592
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuroscience.2009.09.040"; journalName: "Neuroscience"; 
            date: "2010-01-13- 13"; volume: "165"; issue: "1"; pages: "79-89"
COMMENT     Sequence Label: pet28bZFCNGA5
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     284..303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     387..406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        387..405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    406..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             445..467
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             513..530
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             534..566
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             946..963
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1016..1034)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      481 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta
      541 gcatgactgg tggacagcaa atgggtcggg atccagatgc taagaaagtt ctggaagaga
      601 agggaagaca aattcttata aaagagggtt tgctcgagga ggtggaggca gacggaatag
      661 gggtggaaga tattgagaaa agagtagaga ggatggaaga tactattgaa atattacaaa
      721 ctcgatttgc tcgactattg gctcaatata cagcaactca gcagcacctg aagcagagac
      781 ttaccactct ggaatgcaga accaatcatt ctggcagtgg attcctttct gatggtaatg
      841 aaagcactat tgatggggat ggtacaaaca gtgaggtcaa cattcacctt taagtgctaa
      901 gcatggaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      961 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1021 gagcaataac tagcataacc ccttgggg
//
