LOCUS       sequence-138314-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for building 3-color reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a ...
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(224..244)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 taaagaacac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
       61 gaagacaaac atcgactctc aaggggacca aaacgaaaaa agacgctcga aagcgtctct
      121 tttctggaat ttggtaccga gagttcatta agctgctaaa gcgtagtttt cgtcgtttgc
      181 tgctttgtat agttcatcca tgcctagtgt aatcccagca gctgttacaa actcaagaag
      241 gaccatgtga tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      301 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      361 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaaattag ctttgattcc
      421 attcttttgt ttgtctgcag tgatgtatac attatctgaa atagcgttgt attccaattt
      481 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      541 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      601 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgctg
      661 tttcatatga tctgggtatc tagcaaagca ttgaacaccc caagtcaaag tagtgacaag
      721 tgttggccat ggaacaggta gttttccagt agtgcaaa
//
