LOCUS       sequence-138320-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TM0848 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     TM0848(1-363)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28b(+)-TM0848
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgcgttgt cccggatgcg gagcagccat tcagttcgat
      121 gacccgaaaa aaccggggta catcccgcgc gaagtctttg aaaaaaggct cgaagaagga
      181 aaggagatac tctgtcagag atgctttcga atgaaacatt acggcaaact ggaaccagtt
      241 gaattcgact gggattttaa aaaccagttg aaatcgtacc ttggaggatt cgatgtagtg
      301 gtctgggtaa tagatatttt cgatttcgaa gggacctaca gagaagatat agcagaaatt
      361 ctgaagggga agagggttat ctacgtcata aacaagatcg atctcctgcc cagagcggtt
      421 acagtgaagg agatcaaaga atgggtgaag aaaagaataa agacgaagaa cacagatgat
      481 ataaggatcg tcagtgctga aaagaatttc ggcctgacat cgcttgtaaa gtatctttca
      541 cggttgaccg acaaagcact ggtggtggga gtaacgaacg taggaaaatc ttctctacta
      601 aacaagatct gcactcatga aaacaccata agtccctttc cagggacaac tctaggtatt
      661 ctcaaaagaa aggtaaaaga agcgaatctg tatctctacg ataccccagg tatcatgaca
      721 agcgatagga ttctcgatct tttggatcct gagtgtcaa
//
