LOCUS       sequence-138334-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Kim HS, Im HN, An DR, Yoon JY, Jang JY, Mobashery S, Hesek D, Lee M,
            Yoo J, Cui M, Choi S, Kim C, Lee NK, Kim SJ, Kim JY, Bang G, Han BW,
            Lee BI, Yoon HJ, Suh SW
  TITLE     The Cell Shape-determining Csd6 Protein from Helicobacter pylori 
            Constitutes a New Family of l,d-Carboxypeptidase.
  JOURNAL   J Biol Chem. 2015 Oct 9;290(41):25103-17. doi: 
            10.1074/jbc.M115.658781. Epub 2015 Aug 25.
  PUBMED    26306031
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.658781"; journalName: "J Biol Chem"; date: 
            "2015-10-9- 9"; volume: "290"; issue: "41"; pages: "25103-17"
COMMENT     Sequence Label: csd6_13-330_W158A/pET28b(+) N-tag
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
       61 ccgcgcggca gccatatggt gggattgaat gctagtgatc gtttgttaga aatcatgcgc
      121 ctttatcaaa aacaaggctt ggaaatggtg ggtcaaaagt tggattctta tttagcggat
      181 aaatcttttt gggcagaaga acttcaaaac aaggacacgg attttggcta ttatcaaaac
      241 aagcagtttt tatttgtggc taataaatcc aagcccagtt tggagtttta tgagatagaa
      301 aataacatgc ttaaaaaaat caacagctct aaagctcttg taggctctaa aaagggcgat
      361 aagactttag agggcgattt ggccacgcct attggagtgt atcgtatcac gcagaaatta
      421 gagcgcttgg atcaatatta tggcgttttg gcttttgtaa cgaattaccc taatttgtat
      481 gataccttga aaaaacgcac cgggcatggc attgcggtgc atggaatgcc tttaaatggc
      541 gatcggaatg aattgaacac caagggctgt attgcgattg aaaacccgct tttaagctct
      601 tatgacaaag tgttaaaagg cgaaaaagcg ttcctcatca cctatgaaga caagtttttc
      661 ccaagcacca aagaagaatt gagcatgatt ttaagctccc tttttcaatg gaaagaagcc
      721 tgggctaggg gcgattttga acgctacatg cgtttttata acc
//
