LOCUS       sequence-138341-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear expression of RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Yin QF, Hu SB, Xu YF, Yang L, Carmichael GG, Chen LL
  TITLE     SnoVectors for nuclear expression of RNA.
  JOURNAL   Nucleic Acids Res. 2015 Jan;43(1):e5. doi: 10.1093/nar/gku1050. Epub
            2014 Nov 5.
  PUBMED    25378317
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-01"; volume: "43(1):e5. doi"; pages: " 
            10.1093/nar/gku1050. Epub 2014 Nov 5"
COMMENT     Sequence Label: pZW1-snoVector
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(344..358)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     primer_bind     complement(644..663)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg taagtctcga gtgagatctt ggaccaatga tgacttccat
      361 acatgcattc cttggaaagc tgaacaaaat gagtgggaac tctgtactat catcttagtt
      421 gaactgaggt ccaccggggg ctaaagcttc gaattctgca gtcgacggta ccaagattgt
      481 gtgtggatcg atgatgactt ccatatatac attccttgga aagctgaaca aaatgagtga
      541 aaactctata ccgtcattct cgtcgaactg aggtccagca cattactcca acagttaatt
      601 aaccgcggtc tctttcttcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      661 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      721 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      781 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      841 ccgccacaac atc
//
