LOCUS       sequence-138356-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artichoke is a protein degradation reporter plasmid in a lentiviral 
            backbone 
            (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.Pur
            oR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Slabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, 
            Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, 
            Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, 
            Scholl C, Frohling S, Golub TR, Fischer ES, Thoma NH, Ebert BL
  TITLE     The CDK inhibitor CR8 acts as a molecular glue degrader that 
            depletes cyclin K.
  JOURNAL   Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. 
            Epub 2020 Jun 3.
  PUBMED    32494016
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2374-x"; journalName: "Nature"; date: "2020-09";
            volume: "585"; issue: "7824"; pages: "293-297"
COMMENT     Sequence Label: Artichoke
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(150..152,153..866)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(150..152,153..863)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(192..213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(453..472)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     800..821
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cggccggtcg aatcaagctt atcgataccg
       61 tcgactccgg aatagccacc atggagacgg acgtctcagc tgaagctgct gcaaaggaag
      121 ctgcagctaa ggaggctgca gctaaggctg tgagcaaggg cgaggagctg ttcaccgggg
      181 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      241 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      301 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      361 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      421 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      481 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      541 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      601 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      661 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      721 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      781 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      841 tcggcatgga cgagctgtac aagtaaatgc atgagtaact gaggatccgc ccct
//
