LOCUS       sequence-138357-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artichoke is a protein degradation reporter plasmid in a lentiviral 
            backbone 
            (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.Pur
            oR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Slabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, 
            Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, 
            Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, 
            Scholl C, Frohling S, Golub TR, Fischer ES, Thoma NH, Ebert BL
  TITLE     The CDK inhibitor CR8 acts as a molecular glue degrader that 
            depletes cyclin K.
  JOURNAL   Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. 
            Epub 2020 Jun 3.
  PUBMED    32494016
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2374-x"; journalName: "Nature"; date: "2020-09";
            volume: "585"; issue: "7824"; pages: "293-297"
COMMENT     Sequence Label: Artichoke
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..867
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(302..320)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     570..589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      121 gatgataata tggccacaac cctggaattc gccaccatgg tgagcaaggg cgaggaggat
      181 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      241 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      301 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      361 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      421 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      481 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      541 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      601 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      661 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      721 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      781 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      841 ggcggcatgg acgagctgta caagtaaact agtaagcttg gcgtaactag atcttgagac
      901 aaatggcagt attcatccac aattttaaaa gaaaa
//
