LOCUS       sequence-138361-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged
            protein to be maintained.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: MSCV-TAP(FLAG-HA) CYLD
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg
       61 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc
      121 aaacaagttt gtacaaaaaa gttggcgcgc ccaccatgag ttcaggctta tggagccaag
      181 aaaaagtcac ttcaccctac tgggaagagc ggatttttta cttgcttctt caagaatgca
      241 gcgttacaga caaacaaaca caaaagctcc ttaaagtacc gaagggaagt ataggacagt
      301 atattcaaga tcgttctgtg gggcattcaa ggattccttc tgcaaaaggc aagaaaaatc
      361 agattggatt aaaaattcta gagcaacctc atgcagttct ctttgttgat gaaaaggatg
      421 ttgtagagat aaatgaaaag ttcacagagt tacttttggc aattaccaat tgtgaggaga
      481 ggttcagcct gtttaaaaac agaaacagac taagtaaagg cctccaaata gacgtgggct
      541 gtcctgtgaa agtacagctg agatctgggg aagaaaaatt tcctggagtt gtacgcttca
      601 gaggacccct gttagcagag aggacagtct ccggaatatt ctttggagtt gaattgctgg
      661 aagaaggtcg tggtcaaggt ttcactgacg gggtgtacca agggaaacag ctttttcagt
      721 gtgatgaaga ttgtggcgtg tttgttgcat tggacaagct agaactcata gaagatgatg
      781 acactgcatt ggaaagtgat tacgcaggtc ctggggacac aatgcaggtc gaacttcctc
      841 ctttggaaat aaactccaga gtttctttga aggttggaga aacaatagaa tctggaacag
      901 ttatattctg tgatg
//
