LOCUS       sequence-13837-e        7355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Covian-Nares JF, Koushik SV, Puhl HL, Vogel SS
  TITLE     Membrane wounding triggers ATP release and dysferlin-mediated 
            intercellular calcium signaling.
  JOURNAL   J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-93.
  PUBMED    20442251
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2010 Jun 1. 123(Pt 11):1884-93."
COMMENT     Sequence Label: DYSF-Venus
FEATURES             Location/Qualifiers
     source          1..7355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             828..839
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(6429..6431,6432..7145)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6471..6492)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6732..6751)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7079..7100
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7268..7349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7305..7324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgtcagatcc gctgcgccac catggatcct actagtggcc tcgagacgcg tgatatctaa
       61 tacgactcac tatagggaga gccaccatgc caactttgta caaaaaagca ggctcactag
      121 tggtaccgtt taaacgatcg gccgccacca tgctgagggt cttcatcctc tatgccgaga
      181 acgtccacac acccgacacc gacatcagcg atgcctactg ctccgcggtg tttgcagggg
      241 tgaagaagag aaccaaagtc atcaagaaca gcgtgaaccc tgtatggaat gagggatttg
      301 aatgggacct caagggcatc cccctggacc agggctctga gcttcatgtg gtggtcaaag
      361 accatgagac gatggggagg aacaggttcc tgggggaagc caaggtccca ctccgagagg
      421 tcctcgccac ccctagtctg tccgccagct tcaatgcccc cctgctggac accaagaagc
      481 agcccacagg ggcctcgctg gtcctgcagg tgtcctacac accgctgcct ggagctgtgc
      541 ccctgttccc gccccctact cctctggagc cctccccgac tctgcctgac ctggatgtag
      601 tggcagacac aggaggagag gaagacacag aggaccaggg actcactgga gatgaggcgg
      661 agccattcct ggatcaaagc ggaggcccgg gggctcccac caccccaagg aaactacctt
      721 cacgtcctcc gccccactac cccgggatca aaagaaagcg aagtgcgcct acatctagaa
      781 agctgctgtc agacaaaccg caggatttcc agatcagggt ccaggtgatc gaggggcgcc
      841 agctgccggg ggtgaacatc aagcctgtgg tcaaggttac cgctgcaggg cagaccaagc
      901 ggacgcggat ccacaaggga aacagcccac tcttcaatga gactcttttc ttcaacttgt
      961 ttgactctcc tggggagctg tttgatgagc ccatctttat cacggtggta gactctcgtt
     1021 ctctcaggac agatgctctc ctcggggagt tccggatgga cgtgggcacc atttacagag
     1081 agccccggca cgcctatctc aggaagtggc tgctgctctc agaccctgat gacttctctg
     1141 ctggggccag aggctacctg aaaacaagcc tttgtgtgct ggggcctggg gacgaagcgc
     1201 ctctggagag aaaagacccc tctgaagaca aggaggacat tgaaagcaac ctgctccggc
     1261 ccacaggcgt agccctgcga ggagcccact tctgcctgaa ggtcttccgg gccgaggact
     1321 tgccgcagat ggacgatgcc gtgatggaca acgtgaaaca gatctttggc ttcgagagta
     1381 acaagaagaa cttggtggac ccctttgtgg aggtcagctt tgcggggaaa atgctgtgca
     1441 gcaagatctt ggagaagacg gccaaccctc agtggaacca gaacatcaca ctgcctgcca
     1501 tgtttccctc catgtgcgaa aaaatgagga ttcgtatcat agactgggac cgcctgactc
     1561 acaatgacat cgtggctacc acctacctga gtatgtcgaa aatctctgcc cctggaggag
     1621 aaatagaaga ggagcctgca ggtgctgtca agccttcgaa agcctcagac ttggatgact
     1681 acctgggctt cctccccact tttgggccct gctacatcaa cctctatggc agtcccagag
     1741 agttcacagg cttcccagac ccctacacag agctcaacac aggcaagggg gaaggtgtgg
     1801 cttatcgtgg ccggcttctg ctctccctgg agaccaagct ggtggagcac agtgaacaga
     1861 aggtggagga ccttcctgcg gatgacatcc tccgggtgga gaagtacctt aggaggcgca
     1921 agtactccct gtttgcggcc ttctactcag ccaccatgct gcaggatgtg gatgatgcca
     1981 tccagtttga ggtcagcatc gggaactacg ggaacaagtt cgacatgacc tgcctgccgc
     2041 tggcctccac cactcagtac agccgtgcag tctttgacgg gtgccactac tactacctac
     2101 cctggggtaa cgtgaaacct gtggtggtgc tgtcatccta ctgggaggac atcagccata
     2161 gaatcgagac tcagaaccag ctgcttggga ttgctgaccg gctggaagct ggcctggagc
     2221 aggtccacct ggccctgaag gcgcagtgct ccacggagga cgtggactcg ctggtggctc
     2281 agctgacgga tgagctcatc gcaggctgca gccagcctct gggtgacatc catgagacac
     2341 cctctgccac ccacctggac cagtacctgt accagctgcg cacccatcac ctgagccaaa
     2401 tcactgaggc tgccctggcc ctgaagctcg gccacagtga gctccctgca gctctggagc
     2461 aggcggagga ctggctcctg cgtctgcgtg ccctggcaga ggagccccag aacagcctgc
     2521 cggacatcgt catctggatg ctgcagggag acaagcgtgt ggcataccag cgggtgcccg
     2581 cccaccaagt cctcttctcc cggcggggtg ccaactactg tggcaagaat tgtgggaagc
     2641 tacagacaat ctttctgaaa tatccgatgg agaaggtgcc tggcgcccgg atgccagtgc
     2701 agatacgggt caagctgtgg tttgggctct ctgtggatga gaaggagttc aaccagtttg
     2761 ctgaggggaa gctgtctgtc tttgctgaaa cctatgagaa cgagactaag ttggcccttg
     2821 ttgggaactg gggcacaacg ggcctcacct accccaagtt ttctgacgtc acgggcaaga
     2881 tcaagctacc caaggacagc ttccgcccct cggccggctg gacctgggct ggagattggt
     2941 tcgtgtgtcc ggagaagact ctgctccatg acatggacgc cggtcacctg agcttcgtgg
     3001 aagaggtgtt tgagaaccag acccggcttc ccggaggcca gtggatctac atgagtgaca
     3061 actacaccga tgtgaacggg gagaaggtgc ttcccaagga tgacattgag tgcccactgg
     3121 gctggaagtg ggaagatgag gaatggtcca cagacctcaa ccgggctgtc gatgagcaag
     3181 gctgggagta tagcatcacc atccccccgg agcggaagcc gaagcactgg gtccctgctg
     3241 agaagatgta ctacacacac cgacggcggc gctgggtgcg cctgcgcagg agggatctca
     3301 gccaaatgga agcactgaaa aggcacaggc aggcggaggc ggagggcgag ggctgggagt
     3361 acgcctctct ttttggctgg aagttccacc tcgagtaccg caagacagat gccttccgcc
     3421 gccgccgctg gcgccgtcgc atggagccac tggagaagac ggggcctgca gctgtgtttg
     3481 cccttgaggg ggccctgggc ggcgtgatgg atgacaagag tgaagattcc atgtccgtct
     3541 ccaccttgag cttcggtgtg aacagaccca cgatttcctg catattcgac tatgggaacc
     3601 gctaccatct acgctgctac atgtaccagg cccgggacct ggctgcgatg gacaaggact
     3661 ctttttctga tccctatgcc atcgtctcct tcctgcacca gagccagaag acggtggtgg
     3721 tgaagaacac ccttaacccc acctgggacc agacgctcat cttctacgag atcgagatct
     3781 ttggcgagcc ggccacagtt gctgagcaac cgcccagcat tgtggtggag ctgtacgacc
     3841 atgacactta tggtgcagac gagtttatgg gtcgctgcat ctgtcaaccg agtctggaac
     3901 ggatgccacg gctggcctgg ttcccactga cgaggggcag ccagccgtcg ggggagctgc
     3961 tggcctcttt tgagctcatc cagagagaga agccggccat ccaccatatt cctggttttg
     4021 aggtgcagga gacatcaagg atcctggatg agtctgagga cacagacctg ccctacccac
     4081 caccccagag ggaggccaac atctacatgg ttcctcagaa catcaagcca gcgctccagc
     4141 gtaccgccat cgagatcctg gcatggggcc tgcggaacat gaagagttac cagctggcca
     4201 acatctcctc ccccagcctc gtggtagagt gtgggggcca gacggtgcag tcctgtgtca
     4261 tcaggaacct ccggaagaac cccaactttg acatctgcac cctcttcatg gaagtgatgc
     4321 tgcccaggga ggagctctac tgccccccca tcaccgtcaa ggtcatcgat aaccgccagt
     4381 ttggccgccg gcctgtggtg ggccagtgta ccatccgctc cctggagagc ttcctgtgtg
     4441 acccctactc ggcggagagt ccatccccac agggtggccc agacgatgtg agcctactca
     4501 gtcctgggga agacgtgctc atcgacattg atgacaagga gcccctcatc cccatccagg
     4561 aggaagagtt catcgattgg tggagcaaat tctttgcctc cataggggag agggaaaagt
     4621 gcggctccta cctggagaag gattttgaca ccctgaaggt ctatgacaca cagctggaga
     4681 atgtggaggc ctttgagggc ctgtctgact tttgtaacac cttcaagctg taccggggca
     4741 agacgcagga ggagacagaa gatccatctg tgattggtga atttaagggc ctcttcaaaa
     4801 tttatcccct cccagaagac ccagccatcc ccatgccccc aagacagttc caccagctgg
     4861 ccgcccaggg accccaggag tgcttggtcc gtatctacat tgtccgagca tttggcctgc
     4921 agcccaagga ccccaatgga aagtgtgatc cttacatcaa gatctccata gggaagaaat
     4981 cagtgagtga ccaggataac tacatcccct gcacgctgga gcccgtattt ggaaagatgt
     5041 tcgagctgac ctgcactctg cctctggaga aggacctaaa gatcactctc tatgactatg
     5101 acctcctctc caaggacgaa aagatcggtg agacggtcgt cgacctggag aacaggctgc
     5161 tgtccaagtt tggggctcgc tgtggactcc cacagaccta ctgtgtctct ggaccgaacc
     5221 agtggcggga ccagctccgc ccctcccagc tcctccacct cttctgccag cagcatagag
     5281 tcaaggcacc tgtgtaccgg acagaccgtg taatgtttca ggataaagaa tattccattg
     5341 aagagataga ggctggcagg atcccaaacc cacacctggg cccagtggag gagcgtctgg
     5401 ctctgcatgt gcttcagcag cagggcctgg tcccggagca cgtggagtca cggcccctct
     5461 acagccccct gcagccagac atcgagcagg ggaagctgca gatgtgggtc gacctatttc
     5521 cgaaggccct ggggcggcct ggacctccct tcaacatcac cccacggaga gccagaaggt
     5581 ttttcctgcg ttgtattatc tggaatacca gagatgtgat cctggatgac ctgagcctca
     5641 cgggggagaa gatgagcgac atttatgtga aaggttggat gattggcttt gaagaacaca
     5701 agcaaaagac agacgtgcat tatcgttccc tgggaggtga aggcaacttc aactggaggt
     5761 tcattttccc cttcgactac ctgccagctg agcaagtctg taccattgcc aagaaggatg
     5821 ccttctggag gctggacaag actgagagca aaatcccagc acgagtggtg ttccagatct
     5881 gggacaatga caagttctcc tttgatgatt ttctgggctc cctgcagctc gatctcaacc
     5941 gcatgcccaa gccagccaag acagccaaga agtgctcctt ggaccagctg gatgatgctt
     6001 tccacccaga atggtttgtg tccctttttg agcagaaaac agtgaagggc tggtggccct
     6061 gtgtagcaga agagggtgag aagaaaatac tggcgggcaa gctggaaatg accttggaga
     6121 ttgtagcaga gagtgagcat gaggagcggc ctgctggcca gggccgggat gagcccaaca
     6181 tgaaccctaa gcttgaggac ccaaggcgcc ccgacacctc cttcctgtgg tttacctccc
     6241 catacaagac catgaagttc atcctgtggc ggcgtttccg gtgggccatc atcctcttca
     6301 tcatcctctt catcctgctg ctgttcctgg ccatcttcat ctacgccttc ccgaactatg
     6361 ctgccatgaa gctggtgaag cccttcagcg aattccccgg gggtaccgct ggaccggtcg
     6421 ccaccgtggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     6481 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6541 cctacggcaa gctgaccctg aagctcatct gcaccaccgg caagctgccc gtgccctggc
     6601 ccaccctcgt gaccaccctc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     6661 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6721 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6781 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6841 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     6901 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     6961 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     7021 accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     7081 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     7141 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     7201 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     7261 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7321 tttcacaaat aaagcatttt tttcactgca ttcta
//
