LOCUS       sequence-138378-        8741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3C protein (truncated at cleavage site P3; ie, 
            long), C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3c(L)-AP-His
FEATURES             Location/Qualifiers
     source          1..8741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5321..5338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5364..5485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5401..5420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5455..5474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5523..5540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5694..6282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5774..5793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6453..7244,7245..7313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7076..7095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7314..7418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7486..7504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7542..7564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7566..7586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7571..7901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7752..7887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7814..7833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8038..8057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8124..8579)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8261..8282)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8473..8492
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8705..8727
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8719..8736
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8720..8736
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcattccg
     1621 ggcgatttgt gtgttggttg gagtatttat ttgttccatt tgtgtacgag gatcttccca
     1681 gccccaagca agagtttatt taacatttga tgagcttcga gaaaccaaaa cctctgagta
     1741 ctttagtctg tcccaccagc agttagacta cagaatattg ctgatggatg aagatcaaga
     1801 ccggatatat gtggggagca aagaccacat cctgtctttg aatatcaaca atatcagtca
     1861 agaacctttg agtgttttct ggccagcatc aacaatcaaa gttgaagagt gcaaaatggc
     1921 tggcaaagat cctacacatg gctgtggaaa tttcgtccgg gtgattcaga cattcaaccg
     1981 tactcacctg tatgtctgtg ggagtggagc gttcagccca gtgtgcacct acctgaaccg
     2041 gggaaggagg tcagaggacc aggtattcat gatcgactct aagtgtgaat ctggcaaagg
     2101 acgatgctct ttcaacccga atgtgaacac tgtgtctgtt atgatcaatg aggaactctt
     2161 ctcaggaatg tatatagact tcatgggaac agatgctgct attttccgaa gtttaactaa
     2221 gaggaatgca gttcgaactg atcaacataa ttcaaaatgg ctgagtgaac ccatgtttgt
     2281 ggacgcacat gtgatcccag atggcactga tccaaatgat gctaaggtct atttcttctt
     2341 caaagaaaga ctgactgaca acaataggag cacaaaacag attcattcca tgattgcaag
     2401 aatatgccct aatgacactg gtggacaacg tagtcttgtc aacaagtgga ccacattctt
     2461 aaaggcaaga cttgtatgct cagtcacaga tgaagatggc ccagagacac attttgatga
     2521 actagaggat gtctttctgc tggaaactga caatccaagg acaacactcg tgtatggcat
     2581 cttcaccaca tcaagctctg tttttaaggg atcggcagtg tgtgtgtatc atttatctga
     2641 tatacagact gtattcaatg ggccctttgc ccacaaggaa gggcccaatc accagctgat
     2701 ctcctatcaa ggtagaatcc catatcctcg cccaggaact tgcccaggag gggcctttac
     2761 acccaatatg agaaccacca aggacttccc agatgacgtt gtcactttta ttcggaacca
     2821 ccctctcatg tacaattcca tctaccccat ccacagaagg cctctgatag tccgcatagg
     2881 cactgactac aagtacacaa agattgctgt ggaccgtgtc aacgctgctg atgggagata
     2941 ccacgttctg tttctgggca cagatcgggg caccgtgcag aaggtcgtag tccttcctac
     3001 caatagctct gccagtgggg aactcatcct ggaggagctg gaagtcttca agaatcatgt
     3061 tcccataaca acaatgaaaa tctcatccaa aaagcaacag ttgtacgtga gctccaatga
     3121 gggggtttcc caagtctctc tgcatcgctg ccatatctac ggcacagcct gtgcggactg
     3181 ctgcttggcg agggatccat actgtgcctg ggatggccac tcttgctcta ggttctaccc
     3241 cactgggaag cggaggagcc gaagacaaga tgtgagacat ggaaacccac tgacacaatg
     3301 ccgagggttc aatctgaaag catacagaaa tgcagctgaa attgttcagt atggagtaag
     3361 aaataacagc actttccttg agtgtgctcc caagtctcca caggcatcta tcaagtggtt
     3421 gctgcagaaa gacaaagaca ggaggaagga ggttaaactg aacgagcgca ttatagctac
     3481 ttcccaagga ctactgattc gctctgttca agattctgac caaggactct accactgcat
     3541 tgccactgag aacagcttca aacagaccat agccaagatc aacttcaaag ttttagattc
     3601 agaaatggtg gccgttgtga cagacaagtg gtccccgtgg acatgggctg gctctgtgag
     3661 ggctctaccc ttccatccaa aggacatcct gggggcattc agccactcgg aaatgcagct
     3721 catcaatcag tactgcaaag acacccggca gcagcagcag ctgggggaag aaccacagaa
     3781 gatgagaggg gactatggca agctgaaggc tctcatcaac agcaggaaaa gcggcgcgcc
     3841 tggatcttcc ggaatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc
     3901 agccgaggcc ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct
     3961 catcatcttc ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa
     4021 agggcagaag aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata
     4081 tgtggctctg tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc
     4141 cacggcctac ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc
     4201 ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc
     4261 caagaaagca gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc
     4321 agccggcacc tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc
     4381 ctcggcccgc caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat
     4441 tgacgtgatc ctaggtgggg gccgaaagta catgtttcgc atgggaaccc cagaccctga
     4501 gtacccagat gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga
     4561 atggctggcg aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc
     4621 ttccctggac ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata
     4681 cgagatccac cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct
     4741 gcgcctgctg agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga
     4801 ccatggtcat catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga
     4861 cgccattgag agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc
     4921 cgaccactcc cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg
     4981 gctggcccct ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg
     5041 tccaggctat gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag
     5101 ccccgagtat cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga
     5161 cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac
     5221 cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct
     5281 ggcgcccccc gccggcacca ccgacgccgc gcacccgggt catcatcacc atcaccatta
     5341 atctagagat cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa
     5401 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5461 tgtccaaact catcaatgta tcttatcatg tctggtcgac tctagctaga ctcttccgct
     5521 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5581 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5641 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5821 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5881 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5941 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6001 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6061 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6121 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6181 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6241 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6301 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6361 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6421 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6481 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6541 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6601 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6661 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6721 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6781 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6841 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6901 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6961 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7021 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7081 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7441 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7501 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7561 ccggaggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
     7621 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7741 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7801 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7861 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7921 tatccatttt cgtcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7981 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     8041 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8101 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     8161 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8221 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8281 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     8341 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     8401 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     8461 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     8521 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     8581 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8641 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8701 ttttcccagt cacgacgttg taaaacgacg gccagtgaat t
//
