LOCUS       sequence-138382-        9530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 2 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to alkaline phosphatase + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn2-AP-His
FEATURES             Location/Qualifiers
     source          1..9530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6110..6127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6153..6274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6190..6209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6244..6263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6312..6329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6483..7071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6563..6582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7242..8033,8034..8102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7865..7884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8103..8207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8275..8293
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8331..8353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8355..8375)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8360..8690
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8541..8676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8603..8622
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8827..8846
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8913..9368)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9050..9071)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9262..9281
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9494..9516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9508..9525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9509..9525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgggagcacc
     1621 ggccaggaaa agggcaagct tgctgctgct gctgctggcc acaatggctc tggtctcctc
     1681 tccaggatgg agtttttccc agggaacccc agctaccttt ggacccgtct ttgaagagca
     1741 acctgttggc ctgctattcc cagaggagtc tgcagaggat caggtgacac tggcgtgccg
     1801 tgcccgagct agccctccag ccacctatag gtggaagatg aacggcacag agatgaacct
     1861 ggaacctggt tcccgtcacc agctgatggg gggcaacctg gtcatcatga gtcccaccaa
     1921 agcacaggat gctggtgtct accagtgcct agcctctaac ccagtaggca ctgtcgtcag
     1981 caaggaagct gtcctccgct tcggctttct acaggaattc tccaaggagg agagagaccc
     2041 cgtgaaaacc cacgagggct ggggggtgat gctgccctgt aacccacctg cccactaccc
     2101 aggtttgtcc taccgctggc tcctcaacga gttccccaac ttcatcccaa cggatgggcg
     2161 tcacttcgtg tcccagacca cagggaacct gtacatcgcc cgcaccaatg cctcggacct
     2221 gggcaactac tcttgcctgg ctaccagcca cctggacttc tccaccaaga gcgtcttcag
     2281 caaattcgcg cagctcaacc tggctgctga agatcctcga ctctttgctc ccagtatcaa
     2341 agcccggttc cccccagaga cgtacgcact ggttgggcag caggtcaccc tggagtgctt
     2401 tgcctttggg aaccctgttc ccaggatcaa gtggcgcaaa gtggatggtt ccttgtcccc
     2461 tcagtggggc acagcagagc ccaccctgca gatccccagt gttagctttg aagatgaggg
     2521 tacctatgaa tgtgaggcag agaactccaa gggccgtgac accgtccagg gacgcatcat
     2581 tgtgcaagct cagcctgagt ggctaaaggt gatctcagac acagaagccg acattggttc
     2641 caacttacgt tggggctgtg ctgcggcagg caagcctcgg cccatggtgc gctggctgag
     2701 aaacggggaa cctctggcct cccagaaccg ggtggaggtc ttggctgggg acctgcgatt
     2761 ctctaagctg aacctggagg actccggcat gtaccagtgt gtggcagaaa acaagcatgg
     2821 caccatctat gccagtgctg agctggctgt acaagctctg gccccagact tcaggcagaa
     2881 ccctgtgagg cggctgatcc ctgcagcccg agggggagag atcagtatcc cgtgccagcc
     2941 ccgtgcggct ccaaaagcta caatactgtg gagcaagggt actgagattc tggggaacag
     3001 taccagagtg actgtcactt tggacggcac cttgattatc agaaacatca gtcgatcgga
     3061 tgaaggcaaa tatacctgct ttgctgagaa cttcatgggc aaagccaaca gtaccgggat
     3121 cctgtctgtg cgagatgcaa cgaagatcac gctagctcct tcgagtgctg acatcaatgt
     3181 gggtgataac ctgaccctac agtgccacgc ctcgcatgac cccactatgg acctcacgtt
     3241 cacctggacc ctggatgact tccctgttga ctttgataag cctggaggtc actaccggag
     3301 agccagtgtg aaggagacca ttggggacct gactatcctg aatgcccagc tacgccacgg
     3361 agggacatac acatgcatgg cccagacagt ggtggatggt gcatccaagg aggccacagt
     3421 cctggtccga ggtcccccag gtcctcctgg gggtgtggtg gtgagagaca ttggagacac
     3481 tactgttcag cttagctgga gtcgtggctt tgacaatcac agccccattg ccaagtacac
     3541 cctgcaagcg cggactccac cctcagggaa atggaagcag gttcggacca atcctgtgaa
     3601 tatcgagggc aacgcggaaa ctgcccaggt gctggggctc atgccttgga tggactatga
     3661 gtttcgggtt tcagctagca acatcttggg cactggggag cccagcggcc cctccagcag
     3721 aatccgcact aaggaagcag tcccctctgt ggcaccatct ggactcagtg gagggggagg
     3781 agcccctgga gagcttacca tcaactggac tcccatgtca cgagagtacc agaatggaga
     3841 cggcttcggc tacctgctgt ccttccgcag gcaaggcagc tccagttggc agacggcccg
     3901 ggtgcccggc gctgataccc agtacttcgt ctacagcaac gatagcatcc atccctacac
     3961 accctttgag gtcaagatcc gaagctacaa tcgccggggg gatgggcccg agagcctcac
     4021 tgccatcgtg tactcagcag aggaagagcc caaggtggcc cctgccaagg tctgggccaa
     4081 gggttcctca tcctcagaga tgaacgtaag ctgggagcct gtgctacaag acatgaacgg
     4141 catcctcctg ggatatgaga ttcgctactg gaaagccggg gacaaagaag ctgccgctga
     4201 ccgagtgagg acggcagggc tagactccag tgcccgagtc accggcctgt accccaacac
     4261 caagtaccat gtaactgtga gggcctacaa cagggctggc accggaccag ctagcccttc
     4321 agctgatgcc atgaccatga agcccccacc acggcgacct cctggcaaca tctcctggac
     4381 tttctcaagt tccagcctca gccttaagtg ggaccctgtg gttcctctcc gaaatgaatc
     4441 tacagtcact ggctacaaga tgttgtatca gaatgacctg cagccaactc ctatgctcca
     4501 cctcaccagc aagaactgga tagaaatacc agtacctgaa gacattggtc acgctctggt
     4561 gcagattcga accacggggc ctggagggga tgggatacca gccgaagtcc acattgtgag
     4621 aaatactagt ggatcttccg gaatcatccc agttgaggag gagaacccgg acttctggaa
     4681 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
     4741 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
     4801 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
     4861 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
     4921 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
     4981 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
     5041 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
     5101 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
     5161 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
     5221 catggacatt gacgtgatcc taggtggggg ccgaaagtac atgtttcgca tgggaacccc
     5281 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
     5341 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct
     5401 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga
     5461 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     5521 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     5581 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     5641 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     5701 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     5761 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     5821 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     5881 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     5941 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     6001 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     6061 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtc atcatcacca
     6121 tcaccattaa tctagagatc ttattaaagc agaacttgtt tattgcagct tataatggtt
     6181 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6241 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgact ctagctagac
     6301 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7081 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7141 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7201 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7261 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7321 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7381 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7441 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7501 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7561 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7621 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7681 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7741 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7801 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7861 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7921 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7981 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8041 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8101 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8221 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8281 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     8341 atgcagctcc cggaggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     8401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8461 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8521 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8581 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     8641 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8701 gagcttgtat atccattttc gtcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8761 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     8821 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8881 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     8941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     9241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     9301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     9361 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9421 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9481 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
//
