LOCUS       sequence-138385-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of tandem high affinity maltose binding protein
            domains linked to SpyCatcher, for solid-phase polyprotein 
            synthesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET21 MBPx-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(148..486)
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     primer_bind     complement(526..549)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgagtgcg gccgcaagct tctattaaat atgagcgtca cctttagttg ctttgccatt
      181 tacagtaacc tgaccttgct cattaactgt aaaggtaata gcagttgcta cctcataacc
      241 gtctggtgct gcggtttcga caaatgtata ttttcctgga tacaggtaga aatctttcac
      301 ttgtccatct gaaatccatg tactaatagt tttaccagat gaatcacgca actccatagt
      361 tgcaccagct aactctttgc cgtcctcatc acgttttgag aatttaatat gggtagcact
      421 atcttcttca attgtcatat caccggactg accttgctca cttgataaac ctgataaggt
      481 atcaaccatg gcaccactac cgcccgaacc cgagctcgaa ttagtctgcg cgtctttcag
      541 ggcttcatcg acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata
      601 ccagaaagcg gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat
      661 agtggcggcc acacgtggat ctttcaccaa ctcttcctcg taagacttca gcgctacggc
      721 acccagcggt ttgtctttat taaccgcttc cagaccttca tcagtcagca gatagttttc
      781 gaggaactct tttgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac
      841 gccaacgaac gg
//
