LOCUS       sequence-138388-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim
            R, Kim SH
  TITLE     Structural and functional characterization of a novel 
            phosphodiesterase from Methanococcus jannaschii.
  JOURNAL   J Biol Chem. 2004 Jul 23. 279(30):31854-62.
  PUBMED    15128743
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jul 23. 279(30):31854-62."
COMMENT     Sequence Label: pLIC.B2.MJ0936
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             147..164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(711..739)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata catatgcacc accaccacca ccacgcagcg gcggcggcca
      181 tgaaaattgg gataatgagc gatacccatg accacttacc caatattaga aaagctatag
      241 agatttttaa tgatgaaaat gttgagactg ttattcattg tggggatttt gttagtttgt
      301 ttgttataaa agagtttgaa aacttaaacg ccaatatcat agccacttat ggaaataacg
      361 atggggagag atgcaaatta aaggaatggt tgaaggatat aaatgaagag aacataattg
      421 atgattttat atcagttgaa attgatgacc taaaattctt tataacacat gggcatcatc
      481 aatctgtttt agagatggct attaaatctg gtttgtatga tgttgttatt tatggacata
      541 cacatgagag ggtttttgag gaggttgatg atgttttagt cataaaccca ggagaatgtt
      601 gcggttactt aactggcatt cctaccattg ggatattaga cactgaaaag aaggagtata
      661 gggagatagt gttagagtag gggcgcaaag gagaagaact gttcactggt gttgtcccaa
      721 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      781 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      841 ctgttccatg gccaacac
//
