LOCUS       sequence-138391-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim
            R, Kim SH
  TITLE     Structural and functional characterization of a novel 
            phosphodiesterase from Methanococcus jannaschii.
  JOURNAL   J Biol Chem. 2004 Jul 23. 279(30):31854-62.
  PUBMED    15128743
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jul 23. 279(30):31854-62."
COMMENT     Sequence Label: pLIC.B2.MJ0936
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(58..768)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(101..121)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     716..744
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagctta
       61 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      121 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg gagccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      301 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtact cgagtttgtg
      361 tccgagaatg tttccatctt ctttaaaatc aatacctttt aactcgatac gattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      481 tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      541 catgtgatcc ggataacggg aaaagcattg aacaccataa gagaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caacaccagt gaacagttct tctcctttgc gcccctactc
      781 taacactatc tccctatact ccttcttttc cagtgtctaa tatcccaatg gtaaggaaat
      841 gcccagttaa gtaa
//
