LOCUS       sequence-138393-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mSin-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: pCDNA-mSin1(1.1)-HA
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatctctcga ccggcaccat ggccttcttg
      121 gacaatccaa ctatcattct agctcatatt cgacagtcac atgtgaccag tgatgacacg
      181 ggaatgtgtg agatggttct cattgatcat gatgttgacc tagagaagat tcatcctcct
      241 tcaatgcctg gagacagtgg gtcagaaatt cagggaagca atggtgagac tcagggctat
      301 gtatatgccc agtcagtcga tattacctca agttgggact ttggtattag aagacgctca
      361 aacacagctc aaagattaga acgactccga aaagagagac aaaaccagat caaatgcaaa
      421 aatattcagt ggaaagaaag aaattctaag caatcagccc aggagttaaa gtcactgttt
      481 gaaaaaaaat ctctcaaaga gaagcctcca atttctggga agcagtcgat attatctgta
      541 cgcctagaac agtgccctct gcagctgaat aaccctttta acgagtattc caaatttgat
      601 ggcaagggtc atgtaggtac aacagcaacc aagaagatcg atgtctacct ccctctgcac
      661 tcgagccagg acagactgct gccaatgacc gtggtgacaa tggccagcgc cagggtgcag
      721 gacctgatcg ggctcatctg ctggcagtat acaagcgaag gacgggagcc gaagctcaat
      781 gacaatgtca gtgcctactg cctgcatatt gctgaggatg atggggaggt ggacaccgat
      841 ttccccccgc tggattccaa tgagcccatt cataagtttg gcttcagtac tttggccctg
      901 gttgaaaagt actcatctcc tggtctgaca tccaaagagt cactctttgt tcgaataaat
      961 gctgctcatg gattctccct tattca
//
