LOCUS       sequence-138395-        8327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema6C protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema6c-AP-His
FEATURES             Location/Qualifiers
     source          1..8327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4907..4924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4950..5071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4987..5006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5041..5060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5109..5126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5280..5868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5360..5379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6039..6830,6831..6899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6662..6681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6900..7004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7072..7090
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7128..7150)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7152..7172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7157..7487
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7338..7473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7400..7419
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7624..7643
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7710..8165)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7847..7868)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8059..8078
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8291..8313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8305..8322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8306..8322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgccgcgtgc
     1621 cccccactcc atgcccctgc tgctcctgct gctgttgctg tcatcgctcc cccaagccca
     1681 ggctgccttt ccccaggacc ccacccctct gttgacctct gaccttcaag gtgcctctcc
     1741 atcatcctgg ttccggggcc tggaggacga tgctgtggct gcggaacttg ggctggactt
     1801 tcagagattc ctgaccttga accggacctt gcttgtggct gcccgggatc acgttttctc
     1861 cttcgatctt caagcccaag aagaagggga ggggctggtg cccaacaagt ttctgacatg
     1921 gaggagtcaa gatatggaga actgtgcagt ccggggaaag ctgacggacg aatgctacaa
     1981 ctacatccgt gttcttgttc cctggaactc gcagacactt cttgcctgtg gaacaaattc
     2041 cttcagccct atgtgccgca gctatgggat aacatctctg caacaggagg gtgaggagct
     2101 gagtgggcag gctcgatgcc cctttgatgc cacccagtcc actgtggcca tctttgcaga
     2161 gggcagtttg tactcagcca cagcagcaga tttccaggcc agtgatgctg tagtttacag
     2221 aagtcttgga cctcagcctc cactccgttc tgcaaagtat gactccaagt ggcttcgaga
     2281 gccacacttt gtctatgcgt tggagcatgg agagcacgtc tacttcttct tccgagaagt
     2341 ctctgtggag gatgcccgct tagggagggt gcagttttcc agggtagccc gggtatgtaa
     2401 acgtgacatg ggtggctcac ctcgggcctt ggatcgccac tggacatcct tccttaagct
     2461 gaggctcaac tgctctgtcc ctggggactc taccttctac tttgacgtct tacagtcctt
     2521 aactgggcct gtgaacctgc atggccgctc cgctctcttt ggggtcttca ctactcagac
     2581 caatagcatt cctgggtctg cagtctgcgc cttctaccta gatgatattg aacgtggctt
     2641 tgagggcaag ttcaaggagc agaggagtct ggatggggcc tggactcctg tgtctgagga
     2701 caaggtcccc tcacccaggc cagggtcctg tgcaggtgtg ggtgcagctg cctcgttctc
     2761 ctcctctcaa gacctacctg atgatgtcct gctcttcatc aaggcacacc cgctgctgga
     2821 tcctgctgtg ccacccgcca cccatcaacc gctcctcact ctgactagca gggccctgct
     2881 gacccaggta gctgtggacg gaatggctgg tccccacaga aatactactg tcctgtttct
     2941 tggctccaac gatgggacag tgctgaaggt gctacctcca gggggtcagt ctttgggctc
     3001 tgagcctatc gtcttggaag agattgatgc ctacagccat gcccggtgca gtgggaagcg
     3061 gtcaccccga gctgcgcggc ggatcatagg gctggagctg gacactgagg gtcacaggct
     3121 ttttgtggcc tttcctggat gtatcgtcta cctctctctc agccggtgtg cccggcatgg
     3181 agcatgtcag aggagctgcc tggcttctct ggacccatac tgtggatggc atcgatccag
     3241 aggctgcatg agtatcaggg gacctggtgg gactgatgtc gatctgactg ggaaccagga
     3301 atccacagag cacggtgact gccaagatgg agcgaccggg agtcagtctg gccctggaga
     3361 ttctgcttat ggcgtgcgca gggacctttc cccagcctca gcctcccgat ccattcccgg
     3421 cgcgcctgga tcttccggaa tcatcccagt tgaggaggag aacccggact tctggaaccg
     3481 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     3541 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     3601 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     3661 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     3721 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     3781 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     3841 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     3901 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     3961 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     4021 ggacattgac gtgatcctag gtgggggccg aaagtacatg tttcgcatgg gaaccccaga
     4081 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     4141 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat
     4201 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat
     4261 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     4321 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     4381 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     4441 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     4501 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     4561 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     4621 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     4681 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     4741 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     4801 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     4861 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtcatc atcaccatca
     4921 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     4981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     5101 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5161 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5281 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5341 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5401 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5461 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5521 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5581 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5641 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5701 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5761 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5821 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5881 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5941 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6001 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6061 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6121 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6181 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6241 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6301 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6361 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6421 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6481 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6541 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6601 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6661 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6721 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6781 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6841 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6901 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6961 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7021 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7081 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7141 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     7201 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7501 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7561 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7621 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7681 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     7741 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7801 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7861 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7921 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7981 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8041 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8101 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8161 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8221 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8281 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
