LOCUS       sequence-138400-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter
            for cytoplasmic NADP+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Cameron WD, Bui CV, Hutchinson A, Loppnau P, Graslund S, Rocheleau 
            JV
  TITLE     Apollo-NADP: a spectrally tunable family of genetically encoded 
            sensors for NADP
  JOURNAL   Nat Methods. 2016 Feb 15. doi: 10.1038/nmeth.3764.
  PUBMED    26878383
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Feb 15. doi: 10.1038/nmeth.3764."
COMMENT     Sequence Label: Venus-tagged Apollo-NADP+
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(119..832,833..835,836..838))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(164..185)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     513..532
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     772..793
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      835..844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
       61 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg cggccgcttt
      121 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      181 ccatgtgatc gcgcttctcg ttggggtctt tgctcagttt ggactggtag ctcaggtagt
      241 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      301 cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
      361 tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      421 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      481 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      541 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      601 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg
      661 tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt
      721 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      781 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
      841 tggc
//
