LOCUS       sequence-138404-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP coding region followed by enhanced synthetic inhibitory 
            BIC/miR-155 RNA (eSIBR) backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Fowler DK, Williams C, Gerritsen AT, Washbourne P
  TITLE     Improved knockdown from artificial microRNAs in an enhanced miR-155 
            backbone: a designer's guide to potent multi-target RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Nov 17. pii: gkv1246.
  PUBMED    26582923
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 17. pii: gkv1246."
COMMENT     Sequence Label: GFP-eSIBR
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..206)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(189..206)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(226..243)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    232..456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      502..882
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(589..608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     799..820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agtaaggatc cactagtaac ggccgccagt gtgctggaat tcaaagtcga ctggaggctt
       61 gctttgggct gtatgctgtt gtcttcaaga tctggaagac acaggacaca aggcccttta
      121 tcagcactca catggaacaa atggccaccg tgggaggatg acaactcgag cctctagagg
      181 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
      241 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      301 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      361 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaggaca
      421 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      481 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      541 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      601 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      661 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      721 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      781 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      841 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
//
