LOCUS       sequence-138416-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Bacillus subtilis phosphoribosylformylglycinamidine 
            synthase small subunit (BsPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK100
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             327..344
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(397..415)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      411..458
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      495..923
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(582..601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     792..813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtataaa gtaaaagttt atgtcagctt
       61 aaaagaaagt gtactagatc cacaagggag cgctgtccag catgccttgc acagtatgac
      121 ttacaacgaa gttcaagatg tgcgcatcgg gaaatacatg gagcttacca ttgaaaaatc
      181 tgaccgtgat cttgacgtgc tagtgaaaga aatgtgcgaa aaacttcttg cgaacacagt
      241 gattgaagat tatagatatg aggttgagga ggtagtcgca cagtgagaat tcgagctccg
      301 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      361 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      421 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      481 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      841 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      901 ttaacaaaaa tttaacgcga attttaacaa aatatt
//
