LOCUS       sequence-138417-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Bacillus subtilis phosphoribosylformylglycinamidine 
            synthase small subunit (BsPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK100
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             349..371
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(402..421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(403..421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattctcac tgtgcgacta cctcctcaac ctcatatcta
      121 taatcttcaa tcactgtgtt cgcaagaagt ttttcgcaca tttctttcac tagcacgtca
      181 agatcacggt cagatttttc aatggtaagc tccatgtatt tcccgatgcg cacatcttga
      241 acttcgttgt aagtcatact gtgcaaggca tgctggacag cgctcccttg tggatctagt
      301 acactttctt ttaagctgac ataaactttt actttataca tatgtatatc tccttcttaa
      361 agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt
      421 aatttcgcgg gatcgagatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
      481 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
      541 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
      601 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
      661 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
      721 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
      781 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
      841 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
      901 acag
//
