LOCUS       sequence-138425-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses raptor tagged with mCherry and HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: pRK5-HA-mCherry-raptor
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tataagctgc ataacagttg ggccatggcg gccaagctgg ggatccttac ttacttagcg
       61 gccgcccttt tttttttttt tttgagtctg cgcgtgttgt ttactgttgg tcactctctg
      121 ctcttagctt cacattacca tttcgtgttt tgtcaaagtc cgtctcaatt cgcgtttggt
      181 aaaataaata gataatgagc ctctaggccg gcgctctctc gagccggtta acgtcgtgat
      241 tgaattttca catcagacct gtgaagacag cccacgtgca ggcggcacgc agtgcccatg
      301 ggggtgggct ccagggtgga gcgggggcgg gggggggggt cttgacccct ctggagccac
      361 ctgccttttg acaggtggtg attttgtctc aaaacaaacg gaaacgcgtg ttgggtaaga
      421 attgagttga aggactggat gggaagctgc gtgtgcgggt ggagctgggc ttctggaaga
      481 ttctggtgtt gccgcctggc acgccctgtg agctgagtga ggccgggact ctctacacct
      541 tccggacccg ggctgctttc tcggcccaga cgtggggatg ggattcgtgc cggaaccttc
      601 ttctggtggg agctcgcatt ccaaaaagga gcagcggagg ggccgcctct gtcctgcctt
      661 gtcacgtccc ggccagcccc agctcagtct ccagctcagc cccctgaggg ccaggtgtgt
      721 ctctggccaa atgtgaccag
//
