LOCUS       sequence-138429-       13935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG 
            tagged, C-terminally 3X-AU1 tagged wtGATA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant
FEATURES             Location/Qualifiers
     source          1..13935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2994..3065
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             4035..4064
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(4943..4967)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5017..6228
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6065..6084
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6287..6904,6905..6910,6911..6946,6947..6949,6950..6985,
                     6986..6988,6989..7024,7025..7033)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7071..7645
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7238..7255)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             7661..8455
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7661..8455
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7715..7734)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8325..8344
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8623..9211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8676..8696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9094..9105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9214..9230)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9215..9231)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9740..9920
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9946..9963)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9952..10176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10222..10650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10309..10328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10519..10540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10659..10679)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10664..10993
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10844..10979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10906..10925
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11041..11088
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11107..11481
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11611..11732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11648..11667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11702..11721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11781..11797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11781..11797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11794..11816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11805..11821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11829..11835,11836..11853,11854..11859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11874..11895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12012..12029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12183..12771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12263..12282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12942..13733,13734..13802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13565..13584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13803..13907)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt atggactaca
     3001 aggatgacga tgacaaggac tacaaggatg acgatgacaa ggactacaag gatgacgatg
     3061 acaagatggc cttgactgac ggcggctggt gcttgccgaa gcgcttcggg gccgcgggtg
     3121 cggacgccag cgactccaga gcctttccag cgcgggagcc ctccacgccg ccttccccca
     3181 tctcttcctc gtcctcctcc tgctcccggg gcggagagcg gggccccggc ggcgccagca
     3241 actgcgggac gcctcagctc gacacggagg cggcggccgg acccccggcc cgctcgctgc
     3301 tgctcagttc ctacgcttcg catcccttcg gggctcccca cggaccttcg gcgcctgggg
     3361 tcgcgggccc cgggggcaac ctgtcgagct gggaggactt gctgctgttc actgacctcg
     3421 accaagccgc gaccgccagc aagctgctgt ggtccagccg cggcgccaag ctgagcccct
     3481 tcgcacccga gcagccggag gagatgtacc agaccctcgc cgctctctcc agccagggtc
     3541 cggccgccta cgacggcgcg cccggcggct tcgtgcactc tgcggccgcg gcggcagcag
     3601 ccgcggcggc ggccagctcc ccggtctacg tgcccaccac ccgcgtgggt tccatgctgc
     3661 ccggcctacc gtaccacctg caggggtcgg gcagtgggcc agccaaccac gcgggcggcg
     3721 cgggcgcgca ccccggctgg cctcaggcct cggccgacag ccctccatac ggcagcggag
     3781 gcggcgcggc tggcggcggg gccgcggggc ctggcggcgc tggctcagcc gcggcgcacg
     3841 tctcggcgcg cttcccctac tctcccagcc cgcccatggc caacggcgcc gcgcgggagc
     3901 cgggaggcta cgcggcggcg ggcagtgggg gcgcgggagg cgtgagcggc ggcggcagta
     3961 gcctggcggc catgggcggc cgcgagcccc agtacagctc gctgtcggcc gcgcggccgc
     4021 tgaacgggac gtaccaccac caccaccacc accaccacca ccatccgagc ccctactcgc
     4081 cctacgtggg ggcgccactg acgcctgcct ggcccgccgg acccttcgag accccggtgc
     4141 tgcacagcct gcagagccgc gccggagccc cgctcccggt gccccggggt cccagtgcag
     4201 acctgctgga ggacctgtcc gagagccgcg agtgcgtgaa ctgcggctcc atccagacgc
     4261 cgctgtggcg gcgggacggc accggccact acctgtgcaa cgcctgcggg ctctacagca
     4321 agatgaacgg cctcagccgg cccctcatca agccgcagaa gcgcgtgcct tcatcacggc
     4381 ggcttggatt gtcctgtgcc aactgtcaca ccacaactac caccttatgg cgcagaaacg
     4441 ccgagggtga acccgtgtgc aatgcttgtg gactctacat gaaactccat ggggtgccgc
     4501 gaccgctagc gatgaagaaa gagggaattc aaaccaggaa acgaaaacct aagaacataa
     4561 ataaatcaaa gacttgctct ggtaatagca ataattccat tcccatgact ccaacttcca
     4621 cctcttctaa ctcagatgat tgcagcaaaa atacttcccc cacaacacaa cctacagcct
     4681 caggggcggg tgccccggtg atgactggtg cgggagagag caccaatccc gagaacagcg
     4741 agctcaagta ttcgggtcaa gatgggctct acataggcgt cagtctcgcc tcgccggccg
     4801 aagtcacgtc ctccgtgcga ccggattcct ggtgcgccct ggccctggcc gacacctacc
     4861 gctacatcga cacctaccgc tacatcgaca cctaccgcta catctaacaa ttggaagact
     4921 aatgcgtttt cgagatatct agacccagct ttcttgtaca aagtggttga taacatctcg
     4981 agccataaag atggttaatt aacccaccca agatctggcc tccgcgccgg gttttggcgc
     5041 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     5101 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     5161 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     5221 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     5281 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     5341 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     5401 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     5461 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     5521 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     5581 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     5641 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     5701 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     5761 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     5821 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     5881 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     5941 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     6001 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     6061 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     6121 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     6181 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac
     6241 cgagctcgga tctccacccc gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga
     6301 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct
     6361 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     6421 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     6481 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     6541 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     6601 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     6661 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     6721 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     6781 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     6841 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     6901 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     6961 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     7021 gctccccggg taactaagta aggatccgcg gccgcactag aggaattccg cccctctccc
     7081 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgtgtttgtc
     7141 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     7201 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7261 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7321 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7381 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7441 tggatagttg tggaaagagt caaatggctc tcctcaagcg tagtcaacaa ggggctgaag
     7501 gatgcccaga aggtacccca ttgtatggga atctgatctg gggcctcggt gcacatgctt
     7561 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     7621 tttcctttga aaaacacgat gataagctta ccggtccacc atgattgaac aagatggatt
     7681 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7741 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7801 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     7861 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7921 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7981 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     8041 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     8101 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8161 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     8221 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8281 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8341 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8401 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgatgtac
     8461 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     8521 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8581 ttgttgttta gtccctccca attcgatatc aagcttatcg ataatcaacc tctggattac
     8641 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8701 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8761 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8821 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8881 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8941 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9001 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9061 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9121 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9181 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     9241 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     9301 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     9361 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9421 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     9481 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     9541 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     9601 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9661 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     9721 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9781 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9841 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9901 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9961 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
    10021 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
    10081 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
    10141 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
    10201 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    10261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    10321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    10381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    10441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    10501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    10561 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    10621 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
    10681 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10741 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10801 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10861 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10921 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10981 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
    11041 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
    11101 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
    11161 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
    11221 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
    11281 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
    11341 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
    11401 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
    11461 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
    11521 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    11581 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    11641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    11761 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11821 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11881 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11941 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    12001 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12061 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    12121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    12541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    12601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    12661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    13561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    13621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    13681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    13741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    13801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13921 aaagtgccac ctgac
//
