LOCUS       sequence-13843-e        2903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2903)
  TITLE     2-series polycistronic destination vectors
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET empty polycistronic destination vector (2D)
FEATURES             Location/Qualifiers
     source          1..2903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     102..119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     103..119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(333..351)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      347..394
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        461..552
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1417..1456
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1487..2075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1976..1995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2229..2246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2261..2400
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2326..2345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2486..2508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2502..2693)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctctaga aggcctcgga tccgggccct gtacaagatc ctgtaaaacg acggccagtg
      121 aattcactcg agtcccgggg cgatcgcagc ggataacaat ttcacatcct gcagggatat
      181 cggcgcgccc gccagggttt tcccagtcgc ggccgctcgc gcacctggtg ccatcgccct
      241 gatagacgtt aattaaagta ctggccggcc acgcgtctcg agagatccgg ctgctaacaa
      301 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      361 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccgacta
      421 gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata
      481 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      541 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      601 ttttgccttc ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac
      661 caattggggg cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag
      721 agttttcgcc ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga
      781 gcagtattat cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat acactattcg
      841 cagaatgact tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg
      901 gtaagagaat tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt
      961 ctgacaacca tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat
     1021 gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga
     1081 gacaccacaa tgcctgcggc aatggcaaca acattacgca aactattaac tggcgaacta
     1141 cttactctgg cttcacggca acaattaata gactggcttg aagcggataa agttgcagga
     1201 ccactactgc gttcggcact tcctgctggc tggtttattg ctgataaatc tggggcagga
     1261 gagcgtggtt cacggggtat cattgccgca cttggaccag atggtaagcc ttcccgtatc
     1321 gtagttatct acacgacggg tagtcaggca actatggacg aacgaaatag acagattgct
     1381 gaaatagggg cttcactgat taagcattgg taaaccgata caattaaagg ctccttttgg
     1441 agcctttttt tttggacgga ccggtagaaa agatcaaagg atcttcttga gatccttttt
     1501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1621 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2161 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2221 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     2281 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
     2341 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2401 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2461 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2521 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     2581 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     2641 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     2701 tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacga gagaggatgc
     2761 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     2821 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     2881 tcgttaatac agatgtaggt gtt
//
