LOCUS       sequence-138434-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with a single
            serine#33 to alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S33A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..77
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 ccatggacta caaggatgac gatgacaagg actacaagga tgacgatgac aaggactaca
       61 aggatgacga tgacaagatg gccttgactg acggcggctg gtgcttgccg aagcgcttcg
      121 gggccgcggg tgcggacgcc agcgactcca gagcctttcc agcgcgggag cccgccacgc
      181 cgccttcccc catctcttcc tcgtcctcct cctgctcccg gggcggagag cggggccccg
      241 gcggcgccag caactgcggg acgcctcagc tcgacacgga ggcggcggcc ggacccccgg
      301 cccgctcgct gctgctcagt tcctacgctt cgcatccctt cggggctccc cacggacctt
      361 cggcgcctgg ggtcgcgggc cccgggggca acctgtcgag ctgggaggac ttgctgctgt
      421 tcactgacct cgaccaagcc gcgaccgcca gcaagctgct gtggtccagc cgcggcgcca
      481 agctgagccc cttcgcaccc gagcagccgg aggagatgta ccagaccctc gccgctctct
      541 ccagccaggg tccggccgcc tacgacggcg cgcccggcgg cttcgtgcac tctgcggccg
      601 cggcggcagc agccgcggcg gcggccagct ccccggtcta cgtgcccacc acccgcgtgg
      661 gttccatgct gcccggccta ccgtaccacc tgcaggggtc gggcagtggg ccagccaacc
      721 acgcgggcgg cgcgggcgcg caccccggct ggcctcaggc ctcggccgac agccctccat
      781 acggcagcgg aggcggcgcg gctggcggcg gggccgcggg gcctggcggc gctggctcag
      841 ccgcggcgca cgtctcggcg cgcttccc
//
