LOCUS       sequence-138438-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK408
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggatgacc acaacagcta aggctcttat
       61 tacagccttc tacaaaaagc ctgcggcaca tgcttattgg ataggttgtt cgcttggtgg
      121 gctggaaggg ctgattgaag caaaacgcta tccagaagat tatgatggca ttgttgctgg
      181 cgcgccccct aacccgattg taaattttaa tgcagaacaa ctgtgggcgg gatggatgag
      241 ctaccataac tccgccttgc gtgtatcaca cgataaattt catttattga atactgctgt
      301 catgaatgct tgtgcctcac ccgttggcaa aaaacagggg tttttggatg aaccggataa
      361 atgcaccttt atgccagatc agcttttgtg caaaggcgca gatacttcat cctgcctcac
      421 agcaaatgag gtgcaggctg ccaaaaatat ttatcaaggc cctataaacc cgcaaacaca
      481 tcaggttatt ttccccggtc cggccaaagg aagtgaagat ggcttttctg ccgatggcaa
      541 agcctttcct gttgcactgg acctattcaa atatgcagca ttccaaaacc cggaatggga
      601 ttggacaacc ctgaaatggg atactgatac agaggaagcc acacgtaagc ttggcccact
      661 tttacatgta gatgataatc tcaccccatt ctttcagcat ggcgggcggc ttctgatgta
      721 tattggctgg aatgatggtc ataaccctga acaattggca ggttattatc agtctctcca
      781 gcataatgcg gggccaaatt atcaggacaa tgtcaggcta atcaccattc caggcatggg
      841 tcattgctat ggtggtgcgg ggtgtgacac atttagcaaa cttggcgcca ttgataactg
      901 ggtaaccgaa aaagaggcgc cagaaacgct tgtggccgcc cgcgtgagtg atggaaagat
      961 tatccgtacg cgcccacttt gtgcttggcc aaaagtggct cgttatgatg gacatggcaa
     1021 tatggatgat gcacgcagtt ttacctgtgc aacaccatat gcccaagaaa aacatgataa
     1081 a
//
