LOCUS       sequence-138441-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est07.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK378
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..166
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1029..1046
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgccaaaa tcagtagtta atcccgaatt
       61 tcttcccact cttgagaaaa tgccatcttt tgatggtctt tcatcgctct cacttccaga
      121 agtgcgagag gtttttctaa catctgtgcg ccttattgaa gggcggcctc tgccagatgt
      181 tgtaacgcgg gaagaatacg tgccggggtt gaataatgcc ccagatgtac gcgtgattgt
      241 ttatacgcca cgtaatgtac agcccaatgc accggccatg gtttatattc atggtggtgg
      301 ccttgtttct ggtcatcctg aagtggatga tcccaaatgc cagaaactgg ccagtgatct
      361 gggctttcag gttttctccg tagattaccg cctggcacca gaagtgaaat atcccggtgc
      421 aattgaagat tgttatgccg tgctgaaatg ggtgcatgac aatgctagca agcttggcat
      481 ctcgcgtgat aaggttgcgg ttgcgggtga aagtgcaggc ggtggtttaa gtgcagcgct
      541 aagcctgatg gcgcgtgaca aaaaagaata caagctggct tttcagttgc tcatttaccc
      601 catgctggat gaccgcactg caataaatag tgatccgggc gccaattttg gtgaatttgt
      661 ctggacacgg tcggccaaca aatatgcatg gcaggcgtat ctgggggata aagcaggtgg
      721 gccgcaaaca ccaccttatg ccgctgcggg ccgcgcaact gatctggcag gtctgccccc
      781 tacttttatt ggtgtgggat caatggatct tttcttgaag gaagatctgg attacgcaac
      841 tcgtctgatg gctgcggctg tgccaacaga agttatggta gtgcccggcg cgtatcatgt
      901 ttttgacatg tatgtgccag atgctgaact ttctcgcagg tttatggatg cctactgcgc
      961 agcccttaaa cgtacactaa atctgtaaga attcgagctc cgtcgacaag cttgcggccg
     1021 cactcgagca ccaccaccac caccactgag atccggctgc t
//
