LOCUS       sequence-138443-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK379
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..169
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             998..1015
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggatgcca aaatcagtag ttaatcccga
       61 attccttccc attcttgaga aaatgccatc ttttgatggt ctttcgtcgc tttcacttcc
      121 tgaagtgcga gaagtttttc taacatctgt gcgccttata gaaggtcggc ctctgccaga
      181 tgttatcacg cgggaagaat acgtaccggg gttgaacaat gccccagatg tgcgtgtggt
      241 tgtttatacg ccgcgtgaag tgcagcccaa tgcgccagct atggtttata ttcatggtgg
      301 cggtcttgtt tctggccatc cagaagtgga tgaccccaaa tgccaaaagc tggctagtga
      361 tctgggcttt cagattttct cgatagatta tcggctggcg cctgaggtca aatatcccgg
      421 tgcaattgaa gactgttacg cagtgctgaa atgggtgcat gaaaacgcca gaaaacttgg
      481 tatttcgcgt gataaggttg ttgttgcggg tgaaagtgca ggtggtggtt taagtgcagc
      541 gctgagcctg atggcgcgtg acaaaaaaga atacaaactg gcttttcagt tgcttattta
      601 ccccatgctg gatgatcgca cggcaataaa cagtgacccg ggcgccaatt ttggcgaatt
      661 tgtctggaca cgttctgcca acaaatacgc atggcaggct tatctgggtg ataaagctgg
      721 cggcccacaa acaccaccat atgcagccgc tggccgtgca actgatctgg caggtttgcc
      781 tcccaccttt attggtgtgg gatcaatgga tctgtttttg aaagaagacc ttgattacgc
      841 aacgcgcctg atggctgcgg ccgtgccaac ggaagttatg gtggtgccgg gtgcgtatca
      901 tgtgtttgat atgtatgtgc cagatgccga actttcccgc aggtttatgg atgcctactg
      961 tgcagcactt aaacgcacat tgaatctgta actcgagcac caccaccacc accactg
//
