LOCUS       sequence-138446-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with a single
            Threonine#34 to alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..76
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 catggactac aaggatgacg atgacaagga ctacaaggat gacgatgaca aggactacaa
       61 ggatgacgat gacaagatgg ccttgactga cggcggctgg tgcttgccga agcgcttcgg
      121 ggccgcgggt gcggacgcca gcgactccag agcctttcca gcgcgggagc cctccgcgcc
      181 gccttccccc atctcttcct cgtcctcctc ctgctcccgg ggcggagagc ggggccccgg
      241 cggcgccagc aactgcggga cgcctcagct cgacacggag gcggcggccg gacccccggc
      301 ccgctcgctg ctgctcagtt cctacgcttc gcatcccttc ggggctcccc acggaccttc
      361 ggcgcctggg gtcgcgggcc ccgggggcaa cctgtcgagc tgggaggact tgctgctgtt
      421 cactgacctc gaccaagccg cgaccgccag caagctgctg tggtccagcc gcggcgccaa
      481 gctgagcccc ttcgcacccg agcagccgga ggagatgtac cagaccctcg ccgctctctc
      541 cagccagggt ccggccgcct acgacggcgc gcccggcggc ttcgtgcact ctgcggccgc
      601 ggcggcagca gccgcggcgg cggccagctc cccggtctac gtgcccacca cccgcgtggg
      661 ttccatgctg cccggcctac cgtaccacct gcaggggtcg ggcagtgggc cagccaacca
      721 cgcgggcggc gcgggcgcgc accccggctg gcctcaggcc tcggccgaca gccctccata
      781 cggca
//
