LOCUS       sequence-138448-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK322
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             119..127
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1053..1069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1053..1069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1066..1088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttttgtaag tatctgcttc acgcagtgcc ttaactggca gattccaaca gcagacatgc
       61 ggccaagctg gccacccccc acaatgccaa taacggcatt gggcttaagc ggagaatgta
      121 aggaggtcat ttatcttcgg taatgggaga gttggggaca gaggccgtct gcaatgcgcg
      181 ccatgtttca aggcgtgcgg caagggccgg gttatacagt gcaagaatgg aggcggccag
      241 caaggcggca tttttggcgc cggatgcccc aatagccaaa gtgcccacag gcacgccgcc
      301 gggcatctgc acaatggaaa gcaggctgtc catgccttta agagcgcgag attctactgg
      361 aacacccagc acgggcaggc gtgtccatgc cgcacacata cccggcagat gtgcagctcc
      421 accggcccca gcaataatca cattcaggcc gcgttcggct gccgtacgcg cataatccgc
      481 caaccggtct ggcgtacgat gtgctgaaac aatgagggtt tcatgcggaa tttccagctc
      541 tgtcagcaat gcgtctgcat ggcgcatggt ttcccagtct gactggctgc ccatgataat
      601 gcccacaacg ggggcagagg ctgctttatc ttctaaagca ctggaagcag agggcagggg
      661 ggcggtttcg ctcacggtgg gtatctatcc ggttttttaa agtctgtaac ggagaggttc
      721 aggcaatgtc atcagggatc agaaggcttt caaccttggt aatctgatct tttagcagaa
      781 gtttacgttt tttcagccgc tggagataga gttggtcaat aaaagtagtt tcatgcgcca
      841 tgcggttgat aaccgtatcc agatctctgt gttcactccg gagttcatgc agttgggcaa
      901 gcagtaaatc gcgatctcgc agcatgatgt cctgtctcct tcaccattgg tgcggaatcg
      961 tgcgccgggt gtggcggccc gcaaagtgaa tagcatgcct gcaggtcgac tctagaggat
     1021 ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1081 tatccgctc
//
