LOCUS       sequence-138451-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pIndigoBAC-536 with GalK selection marker
            and lens-specific transgenesis marker dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    161..282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(198..217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     252..271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(682..700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(721..737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(734..756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    745..761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(769..775,776..793,794..799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    814..835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagcgcgccg agggccgcca ccacctgttc ctgtagcggc cggccgcgac tctagatcat
       61 aatcagccat accatatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      121 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      241 gcattctagt tgtggtttgt ccaaactcat caatgtatct taatcgaggg ggggcccggt
      301 acccaattcg ccctatagtg agtctcgaga aaattacgcc ccgccctgcc actcatcgca
      361 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      421 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      481 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      541 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
      601 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaacc tcgagggggg
      661 gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
      721 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      781 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      841 gttgcgctca ctg
//
