LOCUS       sequence-138456-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pIndigoBAC-536 with tetR selection marker
            and lens-specific transgenesis marker dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-IndHom-Tol2-TetR-cryaa-dsRed
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccatggtg gcgaccggtg gatccacagt acaaagatcc ccaaatgatg tgtgccggcc
       61 ttagtggagg cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg
      121 atctctctgg aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg
      181 tggactctga cctgagaatg acagtctagc actgaatggc tcagacaata ctcttgtggc
      241 caactatcac cggaaaataa gtgcctgcca aagcaaacca gaccagatga agctacatgg
      301 ctgtagaacg tcagtattta atgtgttgtg gccacttggt tcaccagatt taagagctaa
      361 tttttttaaa tatcacagat aatcaatcgc tttttttttt tcttgactga aaagcttaga
      421 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
      481 aatgcacact attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat
      541 tctgtgcatt tcacaatttt atgttgaaaa gcatataaat ctggatttat tggatcaaac
      601 tattataaac atataatact gtaagatagt cattacagtt tgaataacta catatgcata
      661 ggctgaaact atttaccaat gcaagtttct ctgaagatcg tttattgcca tattagaatt
      721 cgaagctccc ggggcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
      781 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
      841 ctg
//
