LOCUS       sequence-138460-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with 
            C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) 
            with C-terminal c-myc epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK370
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(100..555)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(237..258)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     449..468
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(634..799)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     720..744
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(947..970)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa
       61 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
      121 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      181 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      241 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      301 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      361 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      421 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      481 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      541 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      601 gcgggcctct tcgctattac gccagctgaa ttggagcgac ctcatgctat acctgagaaa
      661 gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt aaaacctaag
      721 agtcacttta aaatttgtat acacttattt tttttataac ttatttaata ataaaaatca
      781 taaatcataa gaaattcgct tatttagaag tgtcaacaac gtatctacca acgatttgac
      841 ccttttccat cttttcgtaa atttctggca aggtagacaa gccgacaacc ttgattggag
      901 acttgaccaa acctctggcg aagaattgtt aattaagagc tcagatctta tcgtcgtcat
      961 ccttgtaatc catcgatact agtgcgaatt tattgtacca atatacaccc ttggactccc
     1021 tacctttttc aggctcgatg aagatgcatt ccttggcctc tctc
//
