LOCUS       sequence-138461-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA and YFP tagged PRAS40.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: pRK5-HA-YFP-PRAS40
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agttgggcat ggcggccaag ctggggatcc ttacttactt agcggccgct caatatttcc
       61 gcttcagctt ctggaagtcg ctggtgttaa gccgcggccg tggcaggtcc ccgaagacct
      121 gggtgtcctc ggcctctcgc agcaccagcg cgcgcatgct cgccgcgatg cggtccaggt
      181 cgggcgaaga gggcggccca ttctcctcat ctgatgaccg cgcctctgtc ctcttctcct
      241 tgaagcccca gacgggcaca gacacaggca gggacttggc gtactgctgt gtgggtaggg
      301 ctgaggctgg gggcactgag caggtggggg ggccggcggg ggtctcctcg ctcaggctgc
      361 catcatctgt actctcgggg tctgactcac agaagggggg aaggtcctgg agggtggcgt
      421 cctcatccat cacaaagagc cctccattat cactaatgcc cagctgctcc ccggaggtct
      481 ctgtctctgt gggctcatcc tcgtcctcct cgttgtcctc tctggccagg gtaggccggg
      541 gtgggctggg tgtgggactg ggtggctgtg gtgctggtgg gggcgcagga ggccgagcag
      601 cagtggcagc cctgtgggcc agtgcgatgt cgtggaggca acggcgcgct gcctccgcca
      661 gggctcctcg accatgggca ggataggcac aggggcccgg gcggggtggt ggcggcggg
//
