LOCUS       sequence-138463-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pIndigoBAC-536 with tetR selection marker
            and lens-specific transgenesis marker dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-IndHom-Tol2-TetR-cryaa-dsRed
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    160..281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(197..216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     251..270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(681..699)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(720..736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(733..755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    744..760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(768..774,775..792,793..798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    813..834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agcgcgccga gggccgccac cacctgttcc tgtagcggcc ggccgcgact ctagatcata
       61 atcagccata ccatatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      121 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      181 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      241 cattctagtt gtggtttgtc caaactcatc aatgtatctt aatcgagggg gggcccggta
      301 cccaattcgc cctatagtga gtctcgagaa aattacgccc cgccctgcca ctcatcgcag
      361 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      421 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      481 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      541 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
      601 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaacct cgaggggggg
      661 cccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
      721 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      781 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      841 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      901 ggccc
//
