LOCUS       sequence-138471-        5701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and
            C-terminally AU1-tagged human wtGATA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pEN_TT 3XFLAG-wtGATA6-3XAU1
FEATURES             Location/Qualifiers
     source          1..5701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..48
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    67..166
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        198..512
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    277..295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    349..367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    384..402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    420..438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     485..509
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             522..593
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             1563..1592
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(2471..2570)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2682..2701)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2760..2779)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3353..3886)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4075..4080,4081..4097,4098..4103))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4760..5348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5249..5268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      5513..5599
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      join(5691..5701,1..17)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat
      181 tcagtcgact ggatcccgag tttactccct atcagtgata gagaacgtat gtcgagttta
      241 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
      301 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      361 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
      421 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
      481 gcagagctcg tttagtgaac cgtcagatcg ccactagtat ggactacaag gatgacgatg
      541 acaaggacta caaggatgac gatgacaagg actacaagga tgacgatgac aagatggcct
      601 tgactgacgg cggctggtgc ttgccgaagc gcttcggggc cgcgggtgcg gacgccagcg
      661 actccagagc ctttccagcg cgggagccct ccacgccgcc ttcccccatc tcttcctcgt
      721 cctcctcctg ctcccggggc ggagagcggg gccccggcgg cgccagcaac tgcgggacgc
      781 ctcagctcga cacggaggcg gcggccggac ccccggcccg ctcgctgctg ctcagttcct
      841 acgcttcgca tcccttcggg gctccccacg gaccttcggc gcctggggtc gcgggccccg
      901 ggggcaacct gtcgagctgg gaggacttgc tgctgttcac tgacctcgac caagccgcga
      961 ccgccagcaa gctgctgtgg tccagccgcg gcgccaagct gagccccttc gcacccgagc
     1021 agccggagga gatgtaccag accctcgccg ctctctccag ccagggtccg gccgcctacg
     1081 acggcgcgcc cggcggcttc gtgcactctg cggccgcggc ggcagcagcc gcggcggcgg
     1141 ccagctcccc ggtctacgtg cccaccaccc gcgtgggttc catgctgccc ggcctaccgt
     1201 accacctgca ggggtcgggc agtgggccag ccaaccacgc gggcggcgcg ggcgcgcacc
     1261 ccggctggcc tcaggcctcg gccgacagcc ctccatacgg cagcggaggc ggcgcggctg
     1321 gcggcggggc cgcggggcct ggcggcgctg gctcagccgc ggcgcacgtc tcggcgcgct
     1381 tcccctactc tcccagcccg cccatggcca acggcgccgc gcgggagccg ggaggctacg
     1441 cggcggcggg cagtgggggc gcgggaggcg tgagcggcgg cggcagtagc ctggcggcca
     1501 tgggcggccg cgagccccag tacagctcgc tgtcggccgc gcggccgctg aacgggacgt
     1561 accaccacca ccaccaccac caccaccacc atccgagccc ctactcgccc tacgtggggg
     1621 cgccactgac gcctgcctgg cccgccggac ccttcgagac cccggtgctg cacagcctgc
     1681 agagccgcgc cggagccccg ctcccggtgc cccggggtcc cagtgcagac ctgctggagg
     1741 acctgtccga gagccgcgag tgcgtgaact gcggctccat ccagacgccg ctgtggcggc
     1801 gggacggcac cggccactac ctgtgcaacg cctgcgggct ctacagcaag atgaacggcc
     1861 tcagccggcc cctcatcaag ccgcagaagc gcgtgccttc atcacggcgg cttggattgt
     1921 cctgtgccaa ctgtcacacc acaactacca ccttatggcg cagaaacgcc gagggtgaac
     1981 ccgtgtgcaa tgcttgtgga ctctacatga aactccatgg ggtgcccaga ccacttgcta
     2041 tgaaaaaaga gggaattcaa accaggaaac gaaaacctaa gaacataaat aaatcaaaga
     2101 cttgctctgg taatagcaat aattccattc ccatgactcc aacttccacc tcttctaact
     2161 cagatgattg cagcaaaaat acttccccca caacacaacc tacagcctca ggggcgggtg
     2221 ccccggtgat gactggtgcg ggagagagca ccaatcccga gaacagcgag ctcaagtatt
     2281 cgggtcaaga tgggctctac ataggcgtca gtctcgcctc gccggccgaa gtcacgtcct
     2341 ccgtgcgacc ggattcctgg tgcgccctgg ccctggccga cacctaccgc tacatcgaca
     2401 cctaccgcta catcgacacc taccgctaca tctaacaatt ggaagactaa tgcgttttcg
     2461 agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2521 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2581 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2641 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     2701 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     2761 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2821 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2881 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2941 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     3001 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     3061 agtgtccctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     3121 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     3181 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     3241 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     3301 ttttgacgag gggaaattag gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     3361 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     3421 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     3481 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     3541 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     3601 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     3661 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     3721 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     3781 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     3841 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     3901 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     3961 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     4021 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     4081 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     4141 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
     4201 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
     4261 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
     4321 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
     4381 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct actaataggt
     4441 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     4501 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     4561 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     4621 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     4681 agctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca
     5461 tggatctcgg ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa
     5521 acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     5581 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg
     5641 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat
     5701 c
//
