LOCUS       sequence-138476-        2439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L-Lactate inducible promoter from E. coli MG1655.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Bba_K822000
FEATURES             Location/Qualifiers
     source          1..2439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(61..104)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    107..126
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      519..576
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(600..617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(771..1359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(851..870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1542..1636)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1657..2316)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2231..2250
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
       61 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
      121 ttctagaatc tagagacgca taggtctcac tatcacattc ctataggccg agtaaggtgt
      181 tcacgccgca tccggcaaga taaggcgctc tggatcaaca acctaagggc aattctctga
      241 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
      301 ttcgcttatc tgacctctgg ttcacaattt cccaattaaa actcacatca atgttgccaa
      361 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
      421 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
      481 actccgtaca gagacccatg ccgtctcatt agctgcagtc cggcaaaaaa gggcaaggtg
      541 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
      601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1501 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
     1561 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1621 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1681 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1741 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1801 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1861 actcacccag ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata
     1921 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1981 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2041 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2101 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2161 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2221 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2281 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2341 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2401 acctcttacg tgcccgatca actcgagtgc cacctgacg
//
