LOCUS       sequence-138482-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4
            SunTag peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-K560-24xGCN4_v4
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             254..310
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             326..382
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             398..454
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             470..526
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             542..598
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             614..670
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             686..742
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             758..814
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             830..886
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ggccctagaa gaacttgctg tcaattatga tcagaagtct caggaagttg aagacaaaac
       61 taaggaatat gaattgctta gtgatgaatt gaatcaggaa tcggcaactt tagcgagtat
      121 agatgctgag cttcagaaac ttaaggaaat gaccaaccac cagaaaaaac gagcagctga
      181 gatgatggca tctttactaa aaggtaccgg atccaacggt ccgggtggat ctggaggtgg
      241 aggttctgga ggagaagaac ttttgagcaa gaattatcat cttgagaacg aagtggctcg
      301 tcttaagaaa ggttctggca gtggagaaga actgctttca aagaattacc acctggaaaa
      361 tgaggtagct agactgaaaa aggggagcgg aagtggggag gagttgctga gcaaaaatta
      421 tcatttggag aacgaagtag cacgactaaa gaaagggtcc ggatcgggtg aggagttact
      481 ctcgaaaaat tatcatctcg aaaacgaagt ggctcggcta aaaaagggca gtggttctgg
      541 agaagagcta ttatctaaaa actaccacct cgaaaatgag gtggcacgct taaaaaaggg
      601 aagtggcagt ggtgaagagc tactatccaa gaattatcat cttgagaacg aggtagcgcg
      661 tttgaagaag ggttccggct caggagagga actgctctcg aagaactatc atcttgaaaa
      721 tgaggtcgct cgattaaaaa agggatcggg cagtggtgag gaactacttt caaagaatta
      781 ccacctcgaa aacgaagtag ctcgattaaa gaaaggttca gggtcgggtg aagaattact
      841 gagtaaaaat tatcatctgg aaaatgaggt agcgagacta aaaaagggga gtggttctgg
      901 cgaagagttg ctatcgaaaa attatcatct tgagaacgaa gttgctaggc tcaaaaa
//
