LOCUS       sequence-138485-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed Kanamycin (G418) selection maker; 3' tagging vector used to 
            create C-terminal fusion proteins with 1x-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ584 pKAN 1x-mCherry
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..62
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(219..237)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     487..506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(805..821)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(840..858)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
       61 tccggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      121 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      181 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      241 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      301 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      361 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      421 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      481 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      541 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      601 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      661 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      721 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      781 gtaagaattc gatatcaagc ttatcgatac cgtcgacctc gagtcatgta attagttatg
      841 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
      901 ca
//
