LOCUS       sequence-138487-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed Kanamycin (G418) selection maker; 3' tagging vector used to 
            create C-terminal fusion proteins with 2x-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ585 pKAN 2x-mCherry
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..728)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(296..315)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     565..583
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcttgatat cgaattctta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
       61 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
      121 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
      181 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
      241 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
      301 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      361 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      421 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      481 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
      541 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
      601 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      661 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
      721 ctcaccatcg gtcccggtcc cggtccaagc ttcttgtaca gctcgtccat gccgccggtg
      781 gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtggga
//
