LOCUS       sequence-138488-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed Kanamycin (G418) selection maker; 3' tagging vector used to 
            add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA 
            stem-loops to gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ645 pKAN 1x-mCherry-12xPP7 V4
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(39..382)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(452..639)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     560..584
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(665..698)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgg ttgtttatgt tcggatgtga
       61 tgtgagaact gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg
      121 gcaaatccta accttttata tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt
      181 ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc
      241 gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct
      301 aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga
      361 gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag gcaagctaaa
      421 cagatccacc ctgttatccc tagcggatct gccggtagag gtgtggtcaa taagagcgac
      481 ctcatactat acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa
      541 ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac
      601 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tggcagatca
      661 cctaataact tcgtatagca tacattatac gaagttatat taagggttag atctatgcat
      721 ccagcgagcc catatgctct gctggtttct agagtcgacg tgcagccagc gagcccatat
      781 gctctgctgg ttctgcatcc agcgagccca tatgctctgc tggtttctag agtcgacgtg
      841 cagccagcga gcccatatgc tctgctggtt ctgcatccag cgagcccata tgctctgctg
      901 gtttctagag tcgacgtgca gccagcgagc ccatatgctc tgctggttct gcatccagcg
      961 agcccatatg ctctgctgg
//
