LOCUS       sequence-138490-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed Kanamycin (G418) selection maker; 3' tagging vector used to 
            add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ617 pKAN 12xPP7 V4
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..381)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(451..638)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     559..583
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(664..697)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
       61 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
      121 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
      181 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
      241 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
      301 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
      361 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg caagctaaac
      421 agatccaccc tgttatccct agcggatctg ccggtagagg tgtggtcaat aagagcgacc
      481 tcatactata cctgagaaag caacctgacc tacaggaaag agttactcaa gaataagaat
      541 tttcgtttta aaacctaaga gtcactttaa aatttgtata cacttatttt ttttataact
      601 tatttaataa taaaaatcat aaatcataag aaattcgctt atttagaagt ggcagatcac
      661 ctaataactt cgtatagcat acattatacg aagttatatt aagggttaga tctatgcatc
      721 cagcgagccc atatgctctg ctggtttcta gagtcgacgt gcagccagcg agcccatatg
      781 ctctgctggt tctgcatcca gcgagcccat atgctctgct ggtttctaga gtcgacgtgc
      841 agccagcgag cccatatgct ctgctggttc tgcatccagc gagcccatat gctctgctgg
      901 tttctagagt cgacgtgcag ccagcgagcc catatgctct gctggttctg catccagcga
      961 gcccatatgc tctgctgg
//
