LOCUS       sequence-138491-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed Kanamycin (G418) selection maker; 3' tagging vector used to 
            add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ617 pKAN 12xPP7 V4
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    555..588
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      614..801
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(669..693)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atccatgcag aaccagcaga gcatatgggc tcgctggctg cacgtcgact ctagaaacca
      121 gcagagcata tgggctcgct ggatgcagaa ccagcagagc atatgggctc gctggctgca
      181 cgtcgactct agaaaccagc agagcatatg ggctcgctgg atgcagaacc agcagagcat
      241 atgggctcgc tggctgcacg tcgactctag aaaccagcag agcatatggg ctcgctggat
      301 gcagaaccag cagagcatat gggctcgctg gctgcacgtc gactctagaa accagcagag
      361 catatgggct cgctggatgc agaaccagca gagcatatgg gctcgctggc tgcacgtcga
      421 ctctagaaac cagcagagca tatgggctcg ctggatgcag aaccagcaga gcatatgggc
      481 tcgctggctg cacgtcgact ctagaaacca gcagagcata tgggctcgct ggatgcatag
      541 atctaaccct taatataact tcgtataatg tatgctatac gaagttatta ggtgatctgc
      601 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
      661 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
      721 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
      781 tattgaccac acctctaccg gcagatccgc tagggataac agggtggatc tgtttagctt
      841 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      901 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      961 catccccatg tataatcatt t
//
