LOCUS       sequence-138499-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein 
            under the MET25 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ529 pTRP MET25p PP7-PS-2x-yeGFP
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        128..275
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             276..950
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 ttactatata tataatatag gaagcattta atagaacagc atcgtaatat atgtgtactt
       61 tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa atagcttgtc
      121 accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga attaattcgg
      181 tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag
      241 tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat ttcacaggta
      301 gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc
      361 tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag agaacaattg
      421 acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca
      481 ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg
      541 tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg caagaatacc
      601 aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac
      661 tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag
      721 gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga
      781 gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg
      841 cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg
      901 taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg
      961 agtagtattt atttaagtat tgt
//
