LOCUS       sequence-138504-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Histidine selection; Used to insert a GAL1 promoter and 24 
            repetitive PP7 version 3 RNA stem-loops before GLT1's start codon 
            (5' tagging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             387..401
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     terminator      695..892
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 accagcccaa aaaaagcaaa aacaaactgt tcaggagcgc aaggcgttta tctcccgtat
       61 cactaatgaa actaaaattc aaatcgctat ttcgctgaat ggtggttata ttcaaataaa
      121 agattcgatt cttcctgcaa agaaggatga cgatgtagct tcccaagcta ctcagtcaca
      181 ggtcatcgat attcacacag gtgttggctt tttggatcat atgatccatg cgttggcaaa
      241 acactctggt tggtctctta ttgttgaatg tattggtgac ctgcacattg acgatcacca
      301 tactaccgaa gattgcggta tcgcattagg gcaagcgttc aaagaagcaa tgggtgctgt
      361 ccgtggtgta aaaagattcg gtactgggtt cgcaccattg gatgaggcgc tatcacgtgc
      421 cgtagtcgat ttatctagta gaccatttgc tgtaatcgac cttggattga agagagagat
      481 gattggtgat ttatccactg aaatgattcc acactttttg gaaagtttcg cggaggcggc
      541 cagaattact ttgcatgttg attgtctgag aggtttcaac gatcaccaca gaagtgagag
      601 tgcgttcaag gctttggctg ttgccataag agaagctatt tctagcaatg gcaccaatga
      661 cgttccctca accaaaggtg ttttgatgtg aagtactgac aataaaaaga ttcttgtttt
      721 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
      781 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
      841 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
      901 tcgatactaa cgccgccatc cagagctcca ggttatcagc aacaacacag tcatatccat
      961 tctcaattag ctctaccaca gtgtg
//
