LOCUS       sequence-138505-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Histidine selection; Used to insert a GAL1 promoter and 24 
            repetitive PP7 version 3 RNA stem-loops before GLT1's start codon 
            (5' tagging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger 
            M, Zenklusen D
  TITLE     The nuclear basket mediates perinuclear mRNA scanning in budding 
            yeast.
  JOURNAL   J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
  PUBMED    26694838
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-12-21- 21"; volume: "211"; 
            issue: "6"; pages: "1131-40"
COMMENT     Sequence Label: pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aataccggca ccgtcaccgt tcccatcaga tgagacggca ccacgatgtg tcatattcac
       61 taaaagatat ctagcgtcag taacaatctt gtgagactgt tcaccatgct tatttgctac
      121 gaaaccgaca ccacaagcgt catgttcata atcagggtcg taaagtcctc gtttgtccgg
      181 aatcacattt gcccaagatt tatgaagatg gtgttcatcg ttatagtttt gaattgtccc
      241 accttcgtaa gcttcttcca atggatcgaa attgtctgat ttcaacactg gcatagatct
      301 atctaacaaa attctatcct gggacatgga tctaatgaac ccgggaatac tgcagccagc
      361 gagcccatat gctctgctgg tttctagagt cgacctgcag ggagcgacgc catatcgtct
      421 gctcctttct aggcaattag gtaccttagg atctaatgaa cccgggaata ctgcagccag
      481 cgagcccata tgctctgctg gtttctagag tcgacctgca gggagcgacg ccatatcgtc
      541 tgctcctttc taggcaatta ggtaccttag gatctaatga acccgggaat actgcagcca
      601 gcgagcccat atgctctgct ggtttctaga gtcgacctgc agggagcgac gccatatcgt
      661 ctgctccttt ctaggcaatt aggtacctta ggatctaatg aacccgggaa tactgcagcc
      721 agcgagccca tatgctctgc tggtttctag agtcgacctg cagggagcga cgccatatcg
      781 tctgctcctt tctaggcaat taggtacctt aggatctaat gaacccggga atactgcagc
      841 cagcgagccc atatgctctg ctggtttcta gagtcgacct gcagggagcg acgccatatc
      901 gtctgctcct ttctaggcaa ttaggtacct taggatctaa tgaacccggg
//
