LOCUS       sequence-138523-        8680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Jacob KK, Whittaker J, Stanley FM
  TITLE     Insulin receptor tyrosine kinase activity and phosphorylation of 
            tyrosines 1162 and 1163 are required for insulin-increased prolactin
            gene expression.
  JOURNAL   Mol Cell Endocrinol. 2002 Jan 15. 186(1):7-16.
  PUBMED    11850117
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol. 2002 Jan 15. 186(1):7-16."
COMMENT     Sequence Label: HIR K1030R
FEATURES             Location/Qualifiers
     source          1..8680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        284..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        664..867
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     818..838
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     912..931
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        912..930
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5239..5304
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5434..5454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5726..5860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5763..5782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5817..5836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6098..6233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6639..6656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6810..7398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6890..6909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7569..8360,8361..8429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8192..8211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8430..8534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8602..8620
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8658..8680)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagcga tgtacgggcc agatatacgc gttgacattg attattgact
      301 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      361 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      421 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      481 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      541 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      601 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      661 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      721 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      781 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      841 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      901 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttccttaag gacatgggca
      961 ccgggggccg gcggggggcg gcggccgcgc cgctgctggt ggcggtggcc gcgctgctac
     1021 tgggcgccgc gggccacctg taccccggag aggtgtgtcc cggcatggat atccggaaca
     1081 acctcactag gttgcatgag ctggagaatt gctctgtcat cgaaggacac ttgcagatac
     1141 tcttgatgtt caaaacgagg cccgaagatt tccgagacct cagtttcccc aaactcatca
     1201 tgatcactga ttacttgctg ctcttccggg tctatgggct cgagagcctg aaggacctgt
     1261 tccccaacct cacggtcatc cggggatcac gactgttctt taactacgcg ctggtcatct
     1321 tcgagatggt tcacctcaag gaactcggcc tctacaacct gatgaacatc acccggggtt
     1381 ctgtccgcat cgagaagaac aatgagctct gttacttggc cactatcgac tggtcccgta
     1441 tcctggattc cgtggaggat aattacatcg tgttgaacaa agatgacaac gaggagtgtg
     1501 gagacatctg tccgggtacc gcgaagggca agaccaactg ccccgccacc gtcatcaacg
     1561 ggcagtttgt cgaacgatgt tggactcata gtcactgcca gaaagtttgc ccgaccatct
     1621 gtaagtcaca cggctgcacc gccgaaggcc tctgttgcca cagcgagtgc ctgggcaact
     1681 gttctcagcc cgacgacccc accaagtgcg tggcctgccg caacttctac ctggacggca
     1741 ggtgtgtgga gacctgcccg cccccgtact accacttcca ggactggcgc tgtgtgaact
     1801 tcagcttctg ccaggacctg caccacaaat gcaagaactc gcggaggcag ggctgccacc
     1861 agtacgtcat tcacaacaac aagtgcatcc ctgagtgtcc ctccgggtac acgatgaatt
     1921 ccagcaactt gctgtgcacc ccatgcctgg gtccctgtcc caaggtgtgc cacctcctag
     1981 aaggcgagaa gaccatcgac tcggtgacgt ctgcccagga gctccgagga tgcaccgtca
     2041 tcaacgggag tctgatcatc aacattcgag gaggcaacaa tctggcagct gagctagaag
     2101 ccaacctcgg cctcattgaa gaaatttcag ggtatctaaa aatccgccga tcctacgctc
     2161 tggtgtcact ttccttcttc cggaagttac gtctgattcg aggagagacc ttggaaattg
     2221 ggaactactc cttctatgcc ttggacaacc agaacctaag gcagctctgg gactggagca
     2281 aacacaacct caccatcact caggggaaac tcttcttcca ctataacccc aaactctgct
     2341 tgtcagaaat ccacaagatg gaagaagttt caggaaccaa ggggcgccag gagagaaacg
     2401 acattgccct gaagaccaat ggggaccagg catcctgtga aaatgagtta cttaaatttt
     2461 cttacattcg gacatctttt gacaagatct tgctgagatg ggagccgtac tggccccccg
     2521 acttccgaga cctcttgggg ttcatgctgt tctacaaaga ggccccttat cagaatgtga
     2581 cggagttcga cgggcaggat gcgtgtggtt ccaacagttg gacggtggta gacattgacc
     2641 cacccctgag gtccaacgac cccaaatcac agaaccaccc agggtggctg atgcggggtc
     2701 tcaagccctg gacccagtat gccatctttg tgaagaccct ggtcaccttt tcggatgaac
     2761 gccggaccta tggggccaag agtgacatca tttatgtcca gacagatgcc accaacccct
     2821 ctgtgcccct ggatccaatc tcagtgtcta actcatcatc ccagattatt ctgaagtgga
     2881 aaccaccctc cgaccccaat ggcaacatca cccactacct ggttttctgg gagaggcagg
     2941 cggaagacag tgagctgttc gagctggatt attgcctcaa agggctgaag ctgccctcga
     3001 ggacctggtc tccaccattc gagtctgaag attctcagaa gcacaaccag agtgagtatg
     3061 aggattcggc cggcgaatgc tgctcctgtc caaagacaga ctctcagatc ctgaaggagc
     3121 tggaggagtc ctcgtttagg aagacgtttg aggattacct gcacaacgtg gttttcgtcc
     3181 ccagaaaaac ctcttcaggc actggtgccg aggaccctag gccatctcgg aaacgcaggt
     3241 cccttggcga tgttgggaat gtgacggtgg ccgtgcccac ggtggcagct ttccccaaca
     3301 cttcctcgac cagcgtgccc acgagtccgg aggagcacag gccttttgag aaggtggtga
     3361 acaaggagtc gctggtcatc tccggcttgc gacacttcac gggctatcgc atcgagctgc
     3421 aggcttgcaa ccaggacacc cctgaggaac ggtgcagtgt ggcagcctac gtcagtgcga
     3481 ggaccatgcc tgaagccaag gctgatgaca ttgttggccc tgtgacgcat gaaatctttg
     3541 agaacaacgt cgtccacttg atgtggcagg agccgaagga gcccaatggt ctgatcgtgc
     3601 tgtatgaagt gagttatcgg cgatatggtg atgaggagct gcatctctgc gtctcccgca
     3661 agcacttcgc tctggaacgg ggctgcaggc tgcgtgggct gtcaccgggg aactacagcg
     3721 tgcgaatccg ggccacctcc cttgcgggca acggctcttg gacggaaccc acctatttct
     3781 acgtgacaga ctatttagac gtcccgtcaa atattgcaaa aattatcatc ggccccctca
     3841 tctttgtctt tctcttcagt gttgtgattg gaagtattta tctattcctg agaaagaggc
     3901 agccagatgg gccgctggga ccgctttacg cttcttcaaa ccctgagtat ctcagtgcca
     3961 gtgatgtgtt tccatgctct gtgtacgtgc cggacgagtg ggaggtgtct cgagagaaga
     4021 tcaccctcct tcgagagctg gggcagggct ccttcggcat ggtgtatgag ggcaatgcca
     4081 gggacatcat caagggtgag gcagagaccc gcgtggcggt tagacggtca acgagtcagc
     4141 cagtctccga gagcggattg agttcctcaa tgaggcctcg gtcatgaagg gcttcacctg
     4201 ccatcacgtg gtgcgcctcc tgggagtggt gtccaagggc cagcccacgc tggtggtgat
     4261 ggagctgatg gctcacggag acctgaagag ctacctccgt tctctgcggc cagaggctga
     4321 gaataatcct ggccgccctc cccctaccct tcaagagatg attcagatgg cggcagagat
     4381 tgctgacggg atggcctacc tgaacgccaa gaagtttgtg catcgggacc tggcagcgag
     4441 aaactgcatg gtcgcccatg attttactgt caaaattgga gactttggaa tgaccagaga
     4501 catctatgaa acggattact accggaaagg gggcaagggt ctgctccctg tacggtggat
     4561 ggcaccggag tccctgaagg atggggtctt caccacttct tctgacatgt ggtcctttgg
     4621 cgtggtcctt tgggaaatca ccagcttggc agaacagcct taccaaggcc tgtctaatga
     4681 acaggtgttg aaatttgtca tggatggagg gtatctggat caacccgaca actgtccaga
     4741 gagagtcact gacctcatgc gcatgtgctg gcaattcaac cccaagatga ggccaacctt
     4801 cctggagatt gtcaacctgc tcaaggacga cctgcacccc agctttccag aggtgtcgtt
     4861 cttccacagc gaggagaaca aggctcccga gagtgaggag ctggagatgg agtttgagga
     4921 catggagaat gtgcccctgg accgttcctc gcactgtcag agggaggagg cggggggccg
     4981 ggatggaggg tcctcgctgg gtttcaagcg gagctacgag gaacacatcc cttacacaca
     5041 catgaacgga ggcaagaaaa acgggcggat tctgaccttg cctcggtcca atccttccta
     5101 agtcgaggga tcttccatac ctaccagttc tgcgcctgca ggtcgcggcc gcgactctag
     5161 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     5221 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     5281 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     5341 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     5401 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     5461 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     5521 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     5581 attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5641 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5701 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5821 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ccgccatggt atcaacgcca
     5881 tatttctatt tacagtaggg acctcttcgt tgtgtagcct ggcctccgct tactctggag
     5941 aaaaagaaga gaggcattgt agaggcttcc agaggcaact tgtcaaaaca ggactgcttc
     6001 tatttctgtc acactgtctg gccctgtcac aaggtccagc acctccatac cccctttaat
     6061 aagcagtttg ggaacgggtg cgggtcttac tccgcccatc ccgcccctaa ctccgcccag
     6121 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6241 ttgcaaaaag ctaattcggc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6361 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6421 agaactctgt agcaccgcct acatacctcg ctctgctgaa gccagttacc agtggctgct
     6481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gagatagtta ccggataagg
     6541 cgcagcggtc gggctgaacg gggggttcgt gcactgcatt aatgaatcgg ccaacgcgcg
     6601 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7681 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8521 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8581 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8641 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
//
